离子辐照选育水稻LOX-1缺失回复突变体及其对种子寿命的影响  

Mutation of Rice (Oryza sativa L.) LOX-1-lacking Mutants with Ion Beam Implantation and Study of Their Storability

作　　者：宋美[1,2] 吴跃进[2] 刘斌美[2] 

机构地区：[1]中国科学院离子束生物工程重点实验室,安徽合肥230031 [2]安徽新华学院药学院,安徽合肥230088

出　　处：《激光生物学报》2009年第2期230-235,共6页Acta Laser Biology Sinica

基　　金：国家“863”计划项目(2001AA241018)

摘　　要：为了研究LOX-1缺失对水稻种子储藏寿命的影响，利用低能N^＋离子对LOX-1，LOX-2缺失品种皖鉴2090进行了注入。田间选育显示，M2株系大量分离，M3株系自身分离少，遗传变异在M3代已具有很好的稳定性。按株系收获田间性状一致的株系，获得了127份皖鉴2090后代材料。利用自然老化处理，研究LOX－1缺失对种子发芽率、电导率、游离脂肪酸和脂质过氧化产物等储藏特性的影，结果表明，LOX－1缺失可以显著延缓种子发芽率的降低，减轻细胞膜损伤引起的细胞内容物渗漏，减少游离脂肪酸和脂质过氧化物的积累，提高水稻种子寿命和储藏品质。In this paper, Wanjian 2090 with deficiency of LOX-1 and LOX-2, a rice variety, was irradiated with ion beam to screen LOX-1/2 near-isogenic lines, which had the similar genetic background while enzyme activities of the either LOX-1 or LOX-2 were different. 127 offspring rice lines without LOX-I or LOX-2 activities were achieved. Through the study of effects of LOX-1 deficiency on rice seed storability, it is found that the lacking of it can significantly reduce the deterioration of seed quality and prolong seed longevity.

关 键 词：离子束诱变 水稻 脂肪氧化酶-1 突变体 种子寿命 

分 类 号：Q691[生物学—生物物理学]