淀粉分支酶sbeIIb基因RNA干涉片段转化玉米自交系的研究(英文)  

A Study on Starch Branching sbeIIb Gene RNAi Fragments Transformation of Maize Inbred-lines

作　　者：吴大强[1] 项艳[1] 程备久[2] 

机构地区：[1]安徽农业大学林学与园林学院,安徽合肥230036 [2]安徽农业大学作物生物学省重点实验室,安徽合肥230036

出　　处：《激光生物学报》2009年第2期264-269,共6页Acta Laser Biology Sinica

基　　金：Supported by the National High Technology Research and Development Program(863Program of China)(NO.2006AA10Z1B4 and NO.2008AZ10A408);the Scientific and Technological Key Program of Ministry of Education(206065)

摘　　要：玉米高直链淀粉育种是玉米分子育种的一个重要研究方向。本实验中，首先研究了不同诱导愈伤培养基对再生体系的影响，确定了Ls＋2，4-D2．0mg／L＋L-pro700mg／L＋CH500mg／L＋3％蔗糖为诱导培养基。同时，掏建并验证了含有淀粉分支酶sbellb基因双干涉片段载体和胚乳特异性启动子的表达载体pCAMBIA1301＋Gln＋1620，并转入根癌农杆菌EHA105，以农杆菌转化法转化玉米自交系178。通过PCR检测，5株转化株表现阳性，初步证明了干涉片段已整合入玉米基因组中。High amylose maize （ Zea rnays L. ） breeding is an important field of molecular maize breeding. In this study, through research of the effect of inducing culture, the optimal inducing culture was fixed as LS ＋ 2,4-D 2.0 rag/ L ＋ L-pro 700 mg/L ＋ CH 500 mg/L ＋ 3 % sucrose. Then, we constructed and detected the expression vector pCAMBIA 1 301 ＋ Glu ＋ 1 620 which carries endosperm specific promoter （ Glu promoter from wheat glutenin） and two interferential fragment of the 16 and 20 exon in maize starch branching sbellb gene, transferred into Agrobacterium tumefaciens strain EHA105, and transformed the maize elite inbred line 178 with the Agrobacterium-protocol. Five transgenic plants which were detected by PCR reaction showed positive signal, indicating that interferential fragment was transformed preliminarily into genome of maize.

关 键 词：玉米自交系 淀粉分支酶sbeIIb基因 遗传转化 RNAi表达载体 

分 类 号：Q789[生物学—分子生物学]