抗病毒噬菌体抗体库研究进展  

Research Progress in Phage Antibody Libraries Targeting Various Viruses

作　　者：吴剑[1] 张风华[1] 张冉[2] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081 [2]湖南师范大学医学院,湖南长沙410081

出　　处：《激光生物学报》2009年第2期270-274,205,共6页Acta Laser Biology Sinica

基　　金：湖南省自然科学基金项目(05JJ30065);湖南省卫生厅科研基金项目(2006-247)

摘　　要：噬菌体抗体库技术是基于噬菌体表面展示技术发展起来的一项基因抗体工程新技术。它将含亿万种基因的抗体可变区基因库转化成展示在噬菌体表面的蛋白库，不仅使对于具备所需属性的单克隆抗体筛选能更方便、快速、高效地在体外进行，还开辟了单克隆抗体人源化的新途径，揭开了人类单克隆抗体生产的历史新篇章。本文综述了近年来针对各种病毒的噬菌体抗体库的构建、筛选方法以及其在各种病毒性疾病研究中的最新进展。Phage antibody library technology is a new gene engineering antibody technology based on the phage display technology. It converts libraries of billions of antibody variable genes into populations of variant proteins displayed on the phage surface, which not only makes the screening of antibodies with desirable properties more convenient, rapid and efficient, but also sets up a new way of humanized monoclonal antibody, and a new page of human monoclonal antibody development had been unveiled. This review summarizes the construction and screening methods of the phage antibody libraries targeting different viruses. The application of this technology in reaserch of viral diseases is also discussed.

关 键 词：噬菌体抗体库 噬菌体表面展示技术 病毒 病毒性疾病 

分 类 号：Q934.2[生物学—微生物学]