渗透压对中华绒螯蟹生精细胞体外培养的影响  

Effect of Osmotic Pressure on the Spermatogenic Cells from Eriocheir sinensis in vitro

作　　者：张瑞新[1] 穆淑梅[1] 康现江[1] 郭明申[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2009年第2期193-198,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(C2008000591)

摘　　要：渗透压是影响体外培养细胞生长和存活的重要因子.通过6种渗透压梯度下体外培养的生精细胞的贴壁率、存活率及RNA/DNA值的测定和细胞生长状况实验,研究了渗透压对中华绒螯蟹（Eriocheir sinensis）生精细胞体外培养的影响.实验结果初步确定了中华绒螯蟹生精细胞体外培养的适宜渗透压范围为900~1000 mmol/L.Osmotic pressure is an important effect factor of cell growth and cell survial in vitro. The effect of osmotic pressure on the male germocyte of Eriocheir sinensis in vitro was examined through the cell adherence, cell survial and RNA/DNA ratio of the cell culture in the conditions of different osmotic pressure. We determined the optimal osmotic pressure for these cells in vitro. The optimal osmotic pressure was 900- 1 000 mmol/L.

关 键 词：中华绒螯蟹 渗透压 生精细胞 体外培养 

分 类 号：Q954.44[生物学—动物学]