应用抑制性消减杂交分离板栗短雄花序芽变相关基因  

Isolation of sport-related genes via suppression subtractive hybridization in short-catkin chestnut

作　　者：朱晓琴[1] 沈元月[1] 冯永庆[1] 秦岭[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《果树学报》2009年第3期340-343,共4页Journal of Fruit Science

基　　金：国家自然科学基金(30872039);北京市自然科学基金(6082004);北京市属市管高校人才强教计划创新团队项目

摘　　要：利用抑制性消减杂交技术分离了板栗正常花序与芽变花序之间表达的差异cDNA片段。以芽变板栗雄花序cDNA为试验方(tester),以对照板栗雄花序cDNA为驱动方(driver),构建了一个板栗芽变花序消减文库。菌落PCR显示插入片段主要在100～1000 bp,文库质量良好。通过对文库阳性克隆随机测序共得到了30个有效EST序列。利用Blast同源性比较,其中一些EST可能与板栗雄花序发育中变短的生物学形态形成密切相关,为后续板栗短雄花序芽变相关基因的研究奠定了基础。In order to uncover the mechanism of sport-related genes in short-catkin chestnut,the suppression subtractive hybridization technique was used. One differential expressing cDNA library was constructed with the cDNAs of formal catkin as driver and the cDNAs of mutant catkin as tester. PCR results suggested that the insertion segments were between 100 to 500 bp and the library was suitable for the following work. Clones were randomly selected and sequenced, and 30 unique ESTs were obtained. The ESTs were annotated by similarity to orthologs and paralogs detected with Blastn and Blastx. Some of the ESTs might be related closely with biological development of the sport in short-catkin chestnut,which lays a foundation for the further study on mutant related genes of short-catkin chestnut.

关 键 词：板栗 短雄花序 抑制性消减杂交 CDNA文库 差异筛选 

分 类 号：S664.2[农业科学—果树学]