水稻苗期不同水分胁迫下抗氧化指标的变化  

Effects of Different Water Stress on Antioxidative Index in Rice Hybrid B You 827

作　　者：陈永军[1] 王泽杰[1] 谢崇华[1] 杨国涛[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《西南农业学报》2009年第2期286-289,共4页Southwest China Journal of Agricultural Sciences

基　　金：四川省"十一五"育种攻关项目(2006-YZGG-1)

摘　　要：对籼型杂交水稻B优827苗期进行不同PEG-6000浓度的水分胁迫处理，研究不同水分胁迫下丙二醛（MDA），O2^-产生速率，超氧化物歧化酶（SOD）和过氧化物酶（POD）同功酶的变化。MDA和O2^-产生速率在轻度水分胁迫下随着胁迫时间的延长，其含量逐渐增加。重度水分胁迫下变化与轻度胁迫下变化相似，但是其含量的上升速度明显比轻度快。对中度和重度水分胁迫下0—4d的叶片进行同功酶电泳比较，发现7．5％PEG处理下，第2天开始出现SOD、POD新条带。20％PEG处理下，第4天出现SOD新条带，POD在胁迫的第1天就出现了新同功酶条带，并随着胁迫时间的增加其同功酶条带有增有减。Hybrid rice B You 827 seedlings were treated by PEG-6000 of different concentration to investigate the variations in MDA, O2^- , superoxide dismutase （SOD）and peroxidase （POD） isozymes. The results showed that, in light water stress, the contents of MDA and O2^- increased slowly with the continuance of the stress. In severe water stress ,the contents of MDA and O2^- increased faster than that in light water stress with the continuance of the stress. The isozymes under 7.5 % and 20 % PEG for four days were detected out. new isozymes of SOD and POD began to appear at the second days of 7.5 % PEG. Under 20 % PEG, new SOD isozymes were detected at the fouth day, and POD new isozymes were detected at the first day,which were increased or reduced with the continuance of wate stress.

关 键 词：杂交水稻 B优827 PEG 同功酶 

分 类 号：S511[农业科学—作物学]