亚洲百合种内杂交后代的多态性及其分离  

Polymorphism and Segregation Patterns of the Progenies from the Cross of Asiatic Lily Hybrids

作　　者：瞿素萍[1] 虞泓[2,3] 吴学尉[4] 李永谊[3] 熊丽[4] 

机构地区：[1]农业部花卉产品质量监督检验测试中心(昆明),云南昆明650205 [2]云南大学生命科学院生态遗传学实验室,云南昆明650091 [3]云南英茂生物技术实验室,云南昆明650212 [4]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《西南农业学报》2009年第2期444-448,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑计划(2007BAD45B01);国家自然科学基金(30160073);云南省自然科学基金重点项目(2002C0002P);云南省人才培引项目(2006PY04)

摘　　要：利用7对DALP引物组合对5个亚洲百合杂交组合后代的多态性及分离方式进行了研究。结果表明,筛选出的7对DALP引物共检测出136个位点,5个杂交组合共得到680个位点,其中多态性位点为379个,多态性位点出现的平均频率是55.7%,呈现出的多态性较高;DALP标记在杂交后代中的分离方式有不分离、发生分离和异常分离3种方式。3种分离方式出现的平均频率与数量分别为44.3%、60;41.8%、57;14.1%和19。该研究结果可为进一步构建百合遗传图谱打下良好的基础,并提供了一定的理论依据。The polymorphism and segregation patterns in the progenies from five Cross combinations of Asiatic lily Hybrids were investigated using 7 primer combinations of DALP markers. The results showed that the polymorphism of the DALP markers was high. Totally 136 DALP loci were detected, and 680 loci were detected from the five cross combinations, totally, of which 379 were polymorphie, and the percentage of polymorphic loci （PPB） was 55.7 % on average. The segregation patterns included Non-segregation, segregation and abnormal segregation, and the frequency and number were ,44.3 % ,60,41.8 %, 57,14.1%, 19, respectively. All above were the work base and could provide some theoretical basis for the further work of the construction of lily genetic map.

关 键 词：DALP 亚洲百合杂种系 多态性 分离方式 

分 类 号：S644.1[农业科学—蔬菜学]