奶牛子宫内膜炎主要病原菌的分离鉴定及中药药敏试验  

Isolation and Identification of the Main Pathogens from Endometritis in Dairy Cow and the Sensitivity Test to Chinese Herbal Medicine

作　　者：李正国[1] 宋华容[1] 罗瑞卿[1,2] 关辉[1] 张飞 吴磊[1] 付东杰[1] 周霞[1] 谷新利[4] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆生产建设兵团121团奶牛场,新疆石河子832000 [3]新疆石河子西部牧业奶牛场．新疆石河子832000 [4]石河子大学科技处,新疆石河子832003

出　　处：《西南农业学报》2009年第2期463-466,共4页Southwest China Journal of Agricultural Sciences

基　　金：新疆兵团科技攻关计划(2006GG22)

摘　　要：对新疆石河子地区不同奶牛场的48头患子宫内膜炎奶牛进行了子宫采样和调查,并对采集的样本进行了病原菌分离和中药敏感性试验,结果从样本中共分离出136株主要病原菌,其中葡萄球菌42株,占30.88%,大肠杆菌33株,占24.26%,变形杆菌25株,占18.38%,链球菌17株,占12.50%,沙门氏菌9株,占5.88%,其他细菌11株,占8.09%;中药药敏试验表明,子宫内膜炎各主要致病菌对益母草、当归、川芎、桃仁、五灵脂、甘草等单味药不敏感或低度敏感,对"宫达宁"复方低敏或中度敏感,未测出多数单味药的最小抑菌浓度,"宫达宁"复方的最小抑菌浓度较高。说明"宫达宁"有一定程度的抑菌作用,但直接抑菌作用不强,其药效的发挥可能主要通过调节动物的整体机能实现的。In order to realize the type and distribution of pathogens from endometritis of dairy cow in Shihezi Region and explore the Anti-inflammatory effect of ＇Gong Da-ning＇, 48 dairy cows with endometritis from different regions were selected on sampling survey and medicine sensitivity of uterus＇s pathogens. The result shows that 136 main pathogens were isolated from the samples, of which there were Staphylococcus 42 strains, accounting for 30.88 % ,E. coli 33 strains, accounting for 24.26 %, deformation Bacillus 25 strains, accounting for 18.38 % ,streptococcus 17 strains, accounting for,12.5 %, Salmonella 9 strains , accounting for 5.88 %, and other bacterial 1 strains, accounting for 8.09 %. The results of Chinese medicine sensitivity showed that the major pathogens from endometritis were insensitive or low-sensitive to Herba leonuri, Chinese Angelica, Ligusticum chuanxiong Hort, Peach Seed, Trogopterus Dung and Licorice Roots Northwest Origin, low-sensitive or highly sensitive to ＇ Gong Da-ning＇ compound, did not detect the MIC of most single medicine, and the MIC was high of ＇ Gong Da-ning＇ compound. This suggested that ＇ Gong Da-ning＇ compound had a certain degree of anti-inflammatory , but the direct effect was not strong, so the efficacy of ＇Gong Da-ning＇ compound could be produced by regulating the overall function of the realization.

关 键 词：子宫内膜炎 病原菌 中药药敏试验 

分 类 号：S857.23[农业科学—临床兽医学]