抗人癌胚抗原单克隆抗体的研制及其在免疫组化中的应用  

Preparation of monoclonal antibody to carcinoembryonic antigen and its application in immuno-histochemistry

作　　者：吴萌[1] 闫静辉[1] 张小兵[1] 程华[1] 陈英珠[1] 李春生[1] 李亚璞[1] 

机构地区：[1]河北省生物研究所,河北石家庄050081

出　　处：《河北省科学院学报》2009年第1期47-49,共3页Journal of The Hebei Academy of Sciences

摘　　要：以高纯CEA为抗原,免疫BALB/c小鼠,取其脾细胞和小鼠SP2/0-Ag14骨髓瘤细胞融合,经2次亚克隆,建立3株稳定分泌抗CEA特异性单克隆抗体的杂交瘤细胞株。应用经纯化的该单克隆抗体进行了结肠癌组织切片的免疫组织化学实验,结果显示全部3种抗CEA单克隆抗体对结肠癌亚细胞组分均呈现特异性结合。表明已筛选出适于制作癌胚抗原临床诊断试剂盒所需的特异性单克隆抗体。BALB/c mice were immunized with a pure carcinoembryonic antigen （CEA） as antigen. The immunized murine splencytes were fused with SP2/0-Ag14 myeloma cells. After screening and subcloning twice, three hybridomas that could secrete anti-CEA monoclonal antibody （McAb） stably were obtained. Using the purified McAbs, immuno-histochemistry and specificity experiments on primary colon carcinoma slice were conducted. The results showed that all the three McAbs were specific binding with the subcellular positions of carcinoma cells and could be used in preparing the diagnosis kit for clinical CEA detection.

关 键 词：人癌胚抗原 单克隆抗体 杂交瘤 免疫组化 

分 类 号：R446[医药卫生—诊断学]