健脾理气法调节实验性FD大鼠神经递质表达的研究  

Effect of Jianpi Liqi Method on neurotransmitter of Functional Dyspepsia Rats

作　　者：刘蔚雯[1] 李冀[2] 王皓宇[2] 柴剑波[3] 

机构地区：[1]福建中医学院药学系,350108 [2]厦门大学医学院中医系,361005 [3]黑龙江中医药大学基础医学院,150040

出　　处：《光明中医》2009年第2期209-211,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：黑龙江省科技厅攻关项目资助(No.GC05C31604)

摘　　要：目的:观察健脾理气法对功能性消化不良(FD)大鼠胃肠组织神经递质表达的影响,探讨该法治疗FD的作用机制。方法:采用大鼠胃电节律失常模型,随机分为4组,应用免疫组织化学方法分别检测胃窦及十二指肠组织乙酰胆碱酯酶(AchE)、一氧化氮合酶(NOS)的表达水平。结果:FD大鼠胃窦及十二指肠组织AchE及NOS的表达较正常对照组明显减少(P<0.01)。枳术饮可显著增加胃窦及十二指肠组织AchE、NOS的阳性表达(P<0.05～0.01)。结论:健脾理气法治疗FD的作用机制可能在于调节胃窦及十二指肠神经递质Ach、NO的表达水平。Objective ： To investigate the effect of Jianpi Liqi Method on neuretransmitter in rats with functional dyspepsia and its mechanism. Methods ： The rat model of electrogastric dysrhythmias was used. All model were divided into 4 groups at random. The distributions of acetylcholine esterase （AchE）, nitric oxide synthase （NOS）in the sinus ventriculi and dodecadactylon were observed by immunohistochemistry. Results：Compared with the normal control group, the distributions of AchE and NOS positive nerves decreased significantly （ P 〈 0.01 ） in model group, and increased significantly after treatment by Zhizhu Yin（ P 〈 0.01 - 0.05）. Conclusion：The therapeutic mechanism of Jiaanpi Liqi Method for functional dyspepsia may regulate the expression of Ach and NO in the sinus ventriculi and dodecadactylon.

关 键 词：FD 健脾理气法 ACHE NOS 中医实验医学 

分 类 号：R259[医药卫生—中西医结合]