HPLC法测定黄天颗粒中盐酸小檗碱的含量  

Determination of Berberine Hydrochloride in Huangtian Granules With HPLC Method

作　　者：许锋[1] 彭松[2] 

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]湖北中医学院,湖北武汉430065

出　　处：《中医药导报》2009年第4期91-92,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:测定黄天颗粒中盐酸小檗碱的含量。方法:采用高效液相色谱法,以十八烷基硅烷键合硅胶为填充剂,以乙腈-0.033mol/L磷酸二氢钾(磷酸调pH值至3.5)(75:25)流动相,流速1.0mL/min,检测波长265nm。结果:盐酸小檗碱进样量在0.096-0.48μg范围内线性关系良好,r=0.9996,平均回收率为99.86%(RSD%=1.93%,n=6)。结论:本方法简便,快速,准确,可用于黄天颗粒中盐酸小檗碱的含量测定。Objective： To determine berberine hydrochloride in Huangtain granules. Methods： The HPLC method was uesed, which consisted of octadecylsilane chemically bonded silica as loading agent with acetonitrile-0.033 mol/L KDP（75：25） as mobile phase at the flow rate of 1.0 mL/min, the detection wavelength was 265 nm. Results： Berberine hydrochloride showed a good linear relationship at the range of 0.096-0.48 μg（r=0.9996）, The average recovery was 99.86%, （RSD%=1.93.n=6）. Conclusion： The method are simple, accurate, can be used for the content determination of berberine hydrochloride in Huangtain granule.

关 键 词：高效液相色谱法 黄天颗粒 盐酸小檗碱 含量测定 

分 类 号：R927.2[医药卫生—药学]