5种体色瓯江彩鲤线粒体COII基因的序列差异和遗传标记研究  

Intraspecific DNA sequence polymorphism and genetic markers in the mitochondrial COII gene from five color patterns of Oujiang color carp

作　　者：吕耀平[1,2] 胡则辉[3] 肖建中[1] 王成辉[4] 胡金强[5] 劳沈颖[1] 叶丽平[1] 项松平 刘青娥[1] 

机构地区：[1]丽水学院化学与生命科学学院,浙江丽水323000 [2]浙江大学动物科学学院,浙江杭州310029 [3]浙江省海洋水产研究所,浙江舟山316100 [4]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 [5]都灵大学兽医学院,意大利皮埃蒙特10095 [6]浙江龙泉省级瓯江彩鲤良种场,浙江龙泉323700

出　　处：《浙江大学学报（理学版）》2009年第3期323-327,334,共6页Journal of Zhejiang University（Science Edition）

基　　金：浙江省自然科学基金资助项目(Y307445);浙江省科技厅项目(2007C32016)

摘　　要：利用PCR方法扩增获得瓯江彩鲤(Cyprinus carpio var.color)线粒体DNA的COII基因,测定该基因片段序列.分析了瓯江彩鲤"全红"、"粉玉"、"粉花"、"麻花"和"大花"共20只个体的序列核苷酸位点差异和遗传多态性.结果表明,"大花"和"全红"遗传多样性最为丰富,"粉花"的遗传多样性相对贫乏.在长度为604 bp的基因片段中,共检测到9个多态性核苷酸位点(占1.49%),20只个体具有4种基因型,5种体色各自的平均核苷酸位点差异分别为0.38%、0.2%、0、0.13%和0.47%.UPGMA分子系统聚类树显示,"粉花"、"麻花"和"粉玉"的遗传关系接近;"全红"相对独立成一支,与其他4种体色瓯江彩鲤的遗传关系较远.COII基因可以作为区分三分支群体的遗传标记.The mitochondrial COⅡ gene was PCR proliferated and sequenced from 20 samples obtained from five color patterns of Oujiang color carp, designated as whole red, red with scattered black spots, whole white and white with scattered black spots. A 604 bp long partial gene segment was acquired and used to study the genetic diversity among the five color patterns. Results indicated that the COⅡ gene locus was relatively more polymorphic in the red color with large black patches （RP） and whole red （WR）, while the polymorphism in white color with large black patches （WP） was relatively poor. A total of 9 polymorphic sites and 4 haplotypes were found in the sequences of the 20 samples. The average nucleotide divergence in the five color patterns of Oujiang color carp was 0. 38%, 0.2%, 0, 0.13%, and 0.47%, respectively. The UPGMA phylogenetic tree suggested that WP, red color with small black spots （RS） and whole white （WW） were closest genetically, while WR diverged and could form another relatively independent branch. For these distinct nucleotide differences, COⅡ gene could be suitable as a genetic marker for distinguishing these three branches of different color patterns of Cyprinus carpio vat. color.

关 键 词：瓯江彩鲤 COⅡ基因 序列多态性 遗传标记 

分 类 号：Q24[生物学—细胞生物学]