家兔肝脏^31P MR波谱重复性检测及其影响因素探讨  

Reproducibility and influencing factors of ^31P MR spectroscopy in rabbit liver with two-dimensional chemical shift imaging

作　　者：余日胜[1] 孙建忠[1] 丁文洪[1] 徐秀芳[1] 王志康[1] 

机构地区：[1]浙江大学医学院第二附属医院放射科,杭州310009

出　　处：《中华放射学杂志》2009年第5期544-550,共7页Chinese Journal of Radiology

基　　金：国家自然科学基金资助项目（30770626）;浙江省重大横向课题资助项目（07491020-121）;杭州市科技局科学研究基金资助项目（20052433824）;浙江省医药卫生科学研究基金资助项目（2005A042）

摘　　要：目的探讨MR二维化学位移成像（2DCSI）在家兔肝脏磷代谢物相对量检测中的重复能力及其影响因素。方法对浓度为0．05mol／L的500ml磷酸盐（NaH2PO4）溶液体模及1只家兔在1d内分别进行30次并于次日重复30次2DCSIMR扫描，分析MR机的稳定性及同一个体磷代谢物的重复能力；对30只家兔在2d内各行1次^31P MRS扫描，分析同一群体磷代谢物的重复能力。在每一次家兔检测前均先行体模^31P MRS扫描，测得校正系数，对不同时间段内所检测得的家兔磷代谢物相对值进行校正，获得校正相对值，采用变异系数分析各代谢物相对量检测误差，并采用t检验进行比较。结果（1）体模检测：2d内磷酸盐体模检测误差分别为4．92％和5．12％，2d内磷化合物相对值分别为16．68±0．82和16．56±0．85，差异无统计学意义（t=0．665，P〉0．05）。（2）同一家兔检测：各磷化合物的检测误差在8．04％～34．13％之间，其中以β-ATP重复性最好，误差均在10％以内；不同2d2次检测磷酸单脂（PME）分别为0．88±0．28和0．88±0．30，磷酸二脂（PDE）分别为4．35±0．66和4．35±0．66，无机磷（Pi）分别为0．95±0．30和0．97±0．28，α-ATP分别为5．58±0．60和5．61±0．61，β-ATP分别为2．70±0．22和2．71±0．22，γ-ATP分别为2．20±0．63和2．18±0．44，差异均无统计学意义（P值均〉0．05）。（3）同一群体家兔检测：各磷化合物的检测误差在8．48％～36．21％之间，其中以β-ATP重复性最好，误差均在10％以内，2次检测PME分别为0．84±0．30和0．79±0．28，PDE分别为4．29±0．72和3．94±0．84，Pi分别为0．91±0．28和0．92±0．31，α-ATP分别为5．65±0．66和5．36±0．60，β—ATP分别为2．71±0．23和2．66±0．25，γ—ATP分别为2．07±0．29和1．99±0．37，差异均无统计学意义（P值均〉0．05）。结论在各磷化合物中，β-ATP相对量误Objective To investigate the reproducibility and influencing factors of relative quantification of phosphorus metabolites with two-dimensional chemical shift imaging （2D CSI）in rabbit liver. Methods Using 2D CSI MRS, 500 ml phosphate （ NaH：PO4 ） solution phantom with 0. 05 mol/L concentration and one healthy rabbit were scanned 30 times respectively in one day and rescanned 30 times in the next day, and the stability of MR scanner and reproducibility of within-run and between-days in the same individual were analyzed. Each of thirty rabbits was scanned and rescanned one time respectively in different days, and the reproducibility of between-days in one group was analyzed. The data were statistically analyzed with t tests. Results （ 1 ） Phosphate solution phantom had a good reproducibility of within-run with the coefficient variation （CV） of 4. 92% and 5.12% respectively in different two days. No significant change of phosphorus metabolites was detected in between-days, which was 16.68 ± 0.82 and 16. 56 ± 0. 85 respectively（t = 0. 665, P 〉 0. 05 ）. （2）The CV of metabolites in one healthy rabbit ranged from 8.04% to 34. 13%. Among the metabolites, β-ATP had the best reproducibility with the CV less than 10%. PME was 0. 88 ± 0. 28 and 0. 88 ± 0. 30, PDE was 4. 35 ± 0. 66 and 4. 35 ± 0. 66, Pi was 0. 95 ± 0. 30 and 0. 97 ±0. 28, α-ATP was 5.58 ± 0. 60 and 5.61 ± 0.61, β-ATP was 2. 70 ± 0. 22 and 2. 71 ± 0. 22, γ-ATP was 2. 20 ±0. 63 and 2. 18 ± 0. 44 respectively, no significant changes of metabolites were detected in between-days（P 〉0. 05）. （3） The CV of metabolites in 30 healthy rabbits ranged from 8.48% to 36. 21%. Among the metabolites, β-ATP had the best reproducibility with CV less than 10%. PME was 0. 84 ± 0. 30 and 0. 79 ± 0. 28, PDE was 4. 29 ± 0. 72 and 3. 94 ± 0. 84, Pi was 0. 91 ± 0. 28 and 0. 92 ± 0. 31, α-ATP was 5.65 ± 0. 66 and 5.36 ± 0.60, β-ATP was 2. 71 ± 0. 23 and 2. 66 ± 0. 25, γ-ATP was 2. 07 ± 0. 29 and 1.99 ± 0. 37 respect

关 键 词：肝 磷放射性同位素 磁共振波谱学 动物实验 

分 类 号：R686[医药卫生—骨科学]