高效液相色谱/质谱鉴别中药复方毛冬青颗粒剂的腺苷  

Applications of HPLC-MS in compound ilex pubescens medicinal granules study

作　　者：陈怡禄[1] 李趣嫦[2] 钟鸣 卓仪[1] 郭穗霞[1] 周启仲[1] 邓六勤[1] 欧阳珊[1] 

机构地区：[1]广州市儿童医院,510120 [2]广东省药品检验所,广州510275 [3]广州汉方现代中药研究开发有限公司,广州510240

出　　处：《广州医药》2009年第3期58-59,共2页Guangzhou Medical Journal

基　　金：广东省产业技术研究与开发资金计划项目(2007B080702016)

摘　　要：采用甲醇超声波辅助萃取中药复方毛冬青颗粒剂,并用高效液相色谱/离子阱质谱联用对其成分进行分析研究。色谱柱采用Dikma Diamonsil C18(4.6×250 mm,5μm),流动相采用水和乙腈进行梯度洗脱,质谱采用了电喷雾(ESI)和大气压化学电离(APCI)两种离子源,并同时采用正、负离子电离模式,分析化合物的分子量和通过相关文献检索对照,证实中药复方毛冬青颗粒剂含有腺苷。In this paper, high performance liquid chromatography along with trap mass spectrometry was used to study the extract of compound Ilex pubescens medicinal granules, which was extracted by ultrasound-assisted extraction using methanol. The column was used Dikma Diamonsil Cls （4. 6 × 250 mm, 5 μm） , the mobile phase consisted of waster and acetonitde with a gradient program. The ionization techniques of ESI APCI were used in this work, both positive-and negative-ion modes. In the liquid chromatograms obtained by TIC from positive-and negative-ion HPLC-MS, the molecular weights of 33 main components were determined based on the corresponding positive-and negative-ion mass spectra information. It was confered according to literature and one components of Adenosine.

关 键 词：毛冬青 南板蓝根 大青叶 高效液相色谱/质谱 腺苷 

分 类 号：R286[医药卫生—中药学]