昆明山海棠提取物对移植物抗宿主病小鼠T细胞亚群的影响  

Effects of Tripterygium hypoglaucum(Level) Hutch Extract on T Lymphocyte Subpopulations in Mice with Graft-versus-host Disease

作　　者：李晟[1] 徐开林[2] 李振宇[2] 潘秀英[2] 

机构地区：[1]中国人民解放军空军航空医学研究所附属医院内二科,北京100089 [2]徐州医学院附属医院血液科,江苏徐州221002

出　　处：《广州中医药大学学报》2009年第3期246-250,251,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号:30170389);江苏省中医药管理局项目(编号:H05104)

摘　　要：【目的】观察昆明山海棠(THH)提取物对移植物抗宿主病(GVHD)小鼠外周血T细胞亚群(CD3+CD4+、CD3+CD8+)的影响,探讨THH对GVHD的预防作用及机制。【方法】BALB/c受鼠(H-2d)清髓性预处理后输注C57BL/6供鼠(H-2b)骨髓细胞和脾细胞混合液,随机分4组:模型对照组、环孢素A(CsA)组(剂量为10mg·kg-.1d-1)、THH组(剂量为400mg·kg-.1d-1)、THH+CsA联合用药组(联药组,剂量为THH100mg·kg-.1d-1+CsA5mg·kg-.1d-1)。观察移植后受鼠GVHD发生及存活情况以及嵌合体植入情况;采用流式细胞术测定受鼠外周血CD3+CD4+、CD3+CD8+T细胞百分率。【结果】①CsA组、THH组、联药组受鼠生存时间均长于模型对照组(P<0.01);②CsA组、THH组、联药组受鼠GVHD临床表现和病理改变均较模型对照组轻;③模型对照组、CsA组、THH组、联药组受鼠骨髓细胞上异基因H-2b百分率均>95%;④与模型对照组比较,CsA组、THH组、联药组受鼠移植后外周血CD3+CD4+、CD3+CD8+细胞百分率均下降(P<0.05),CD4+/CD8+细胞比值均上升(P<0.05),联药组变化最显著(P<0.05)。【结论】THH可通过调节CD4+/CD8+细胞比值,抑制T淋巴细胞免疫反应而预防小鼠GVHD,THH和CsA小剂量联合用药可能有协同作用。Objective To observe the preventive effects of Tripterygium hypoglaucum （Level） Hutch （THH） extract on T lymphocyte subpopulations in mice with graft-versus-host disease （GVHD） and to explore its protective mechanism. Methods 2×10^7 bone marrow cells mixed with 2×10^7 spleen cells from C57BL/6 mouse were transplanted into the myeloablative irradiated inbred BALB/c mouse. The experiment groups were designed as follows： model control group, cyclosporine A （ CsA, 10 mg·kg^-1·d^-1 ） group, THH （400 mg·kg^-1·d^-1 ） group, combination group （CsA 5 mg·kg^-1·d^-1 ＋ THH 100 mg·kg^-1·d^-1）. The ocurrenee of GVHD was assessed by signs of weight loss, diarrhea, ruffled fur, hunched posture and histologic changes of skin, liver and small intestines. Chimerism was detected by monitoring the H-2^b molecule in bone marrow cells of recipient mice with flow eytometer. The percentages of CD3^＋ CD4^＋ and CD3^＋ CD8^＋T cells in peripheral blood were detected by flow cytometer. Results The survival time of CsA group, THH group and combination group were prolonged as compared with that of the model control group （P 〈0.01 ）. GVHD signs and histologie changes of CsA group, THH group, and combination group were milder than those of the model control group. Allogeneic H-2^b chimerism rates in the four groups were all higher than 95%. The percentages of CD3^＋CD4^＋ and CD3^＋CD8^＋ T cells in peripheral blood were lower and the ratio of CD4^＋/CD8^＋ T cells was higher in CsA group, THH group and combination group than those in the model control group （ P 〈 0. 05 ）. The above changes were obvious in the combination group. Conclusion THH combined with small-dose CsA has synergistic effect on preventing GVHD through regulating the ratio of CD4^＋/CD8^＋ T cell subpopulations.

关 键 词：昆明山海棠/药理学 移植物抗宿主病/中药疗法 T细胞亚群/血液 皮肤/病理学 肝脏/病理学 小肠/病理学 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]