中药益气化纤汤抗大鼠肺纤维化的实验研究  

Experimental Study of Intervention of Yiqi Huaxian Decoction on Rats Pulmonary Fibrosis

作　　者：蔡望喜[1] 尹杉杉[1] 常建方[1] 

机构地区：[1]湖北省中医院,湖北武汉430061

出　　处：《广州中医药大学学报》2009年第3期263-265,269,316,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】观察益气化纤汤对肺纤维化模型大鼠肺泡巨噬细胞(AM)和肺间质巨噬细胞(IM)中转化生长因子β1(TGF-β1)mRNA表达的影响。【方法】选用Wistar大鼠96只,随机分为假手术组、模型组、益气化纤汤组(剂量为4g·kg-·1d-1)、氢化可的松组(剂量为20mg·kg-·1d-1);采用博莱霉素A5(剂量为6mg/kg)气管注入法复制肺纤维化模型;分别于造模第7、28天分离培养AM、IM,采用分子原位杂交法(ISH)检测TGF-β1 mRNA的表达。【结果】模型组AM、IM中TGF-β1 mRNA表达阳性细胞百分率及积分光密度(D)值显著升高(均P<0.01);益气化纤汤可显著降低AM、IM中TGF-β1 mRNA表达阳性细胞百分率及D值(均P<0.01),其作用与氢化可的松相仿。【结论】益气化纤汤治疗肺纤维化的作用可能与其能降低TGF-β1的表达,抑制纤维化的发展进程有关。Objective To observe the effect of Yiqi Huaxian Decoction （YHD） on transforming growth factor β1 （TGF-β1） mRNA expression in rats alveolar macrophages （AM） and pulmonary interstitial macrophages （IM）. Methods Ninety-six Wistar rats were randomized into sham-operation group, model group, YHD （4 g·kg^-1·d^-1 ） group, and hydrocortisone （20 mg·kg^-1· d^-1 ） group. Bleomycin A5 was injected into the rats＇ trachea to induce pulmonary fibrosis. AM and IM were isolated for the culturing after modeling for 7 and 28 days. Molecular in-situ hybridization （ISH） was used for the detection of TGF-β1 expression in AM and IM. Results Percentage of cells in AM and IM with TGF-β1 positive expression and the score of optic density were increased in the model group （P 〈0. 01 compared with those in sham-operation group） , YHD had an effect on deceasing the above two indexes （ P 〈 0. 01 ） , and its effect was similar to that of hydrocortisone. Conclusion The therapeutic mechanism of YHD is probably related with the decrease of TGF-β1 expression and the inhibition of fibrosis.

关 键 词：益气化纤汤/药理学 肺纤维化/中药疗法 转化生长因子β 肺泡巨噬细胞/病理学 肺间质巨噬细胞/病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]