一种适合AFLP分析的油茶DNA提取方法  

DNA Extraction of AFLP Reaction System for Camellia oleifera

作　　者：黄永芳[1] 廖国春 吴雪辉[1] 安星[1] 阮瑶瑶[1] 谭裕开[1] 

机构地区：[1]华南农业大学经济林研究中心,广东广州510642 [2]广东省肇庆市林业科学研究所

出　　处：《广东林业科技》2009年第1期23-26,共4页Forestry Science and Technology of Guangdong Province

基　　金：广东省自然科学基金项目(06025809);国家"十一五"科技支撑项目(2006BAD18B0204)

摘　　要：以油茶的成熟叶片为材料提取DNA,40个样品用改良的提取纯化方法成功提取出适用于AFLP分析的高质量DNA,OD260/OD280在1.7-2.0之间,可以用于AFLP反应。该方法打破了仅能用幼嫩叶片作为提取材料的限制,具有更广泛的适用性和重要的实用性。DNA of samples was extracted and purified from mature leaves, 40 samples were succeed to extracted high quality DNA by using reformative extraction method. OD260/OD280 value is between 1.7 and 2.0, DNA is suitable for AFLP reaction. This method has broken the limitation of using tender leaves only for DNA extraction, which has wider range applicability and great practical significance.

关 键 词：油茶 DNA提取 AFLP反应 

分 类 号：S794.4[农业科学—林木遗传育种]