两种方法在乙型肝炎病毒检测中的应用比较  

Two methods in the examination of hepatitis B virus

作　　者：魏成翠 贾琳[1] 刘阿玲[1] 

机构地区：[1]沈阳市胸科医院检验科,辽宁沈阳110044

出　　处：《临床和实验医学杂志》2009年第5期9-11,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的调查时间分辨荧光免疫分析技术和胶体金法在乙型肝炎血清学标志物检测中的应用情况。方法采用泰莱———型半自动时间分辨荧光免疫分析仪及配套试剂对96例乙型肝炎患者血清检测乙肝病毒表面抗原(HB sAg)、乙肝病毒表面抗体(HB sAb)、乙肝病毒e抗原(HBeAg)、乙肝病毒e抗体(HBeAb)及乙肝病毒核心抗体(HB cAb),同时用胶体金法进行检测。结果时间分辨荧光免疫分析法(TRF)检测乙型肝炎血清学5项标志物结果同胶体金法比较灵敏度较高,特异性稍差。结论时间分辨荧光免疫分析技术检测乙型肝炎血清学5项标志物,灵敏度高、稳定性好、经济、符合国情,可作为乙型肝炎病毒定量的一种常规方法。Objective To research the application of the analysis technique of the time resolve and performing gold assay in serum testing for the hepatitis B virus. Methods By Tailai - semiautomatictime resolved fluoroimmuno assay instrument and reagents to examine 96serum sampies of patients for HBsAg, HBsAb, HBeAg, HBeAb and HBcAb and then compare. Results The results with One Step Cassete Style HBV Test. Conclusion The method of examining serological markers of hepatitis B virus by time resolved fluoruimmuno assay with high sensitivity and good stability is cost - effective and suitable for China＇s conditions, and can be used as a routine method of quantitative assay for hepatitis B virus.

关 键 词：时间分辨荧光免疫分析 胶体金法 乙型肝炎病毒 检测 

分 类 号：R446.6[医药卫生—诊断学]