肿节风化学成分的高效液相色谱-电喷雾串联质谱分析  

Study on the Chemical Constituents of Sarcandra glabra by HPLC-ESI-MS/MS

作　　者：罗奇志[1] 戴开金[1] 马安德 

机构地区：[1]南方医科大学,广东广州510515

出　　处：《中药材》2009年第4期526-529,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:对比分析肿节风药材及清热消炎宁胶囊水提液乙酸乙酯萃取部位的主要化学成分。方法:采用Zorbax C18柱,以乙腈-0.1%甲酸水溶液梯度洗脱,紫外和电喷雾质谱同时在线检测,全离子扫描模式扫描,对每一组分峰进行质谱分析。结果:通过质谱分析及文献对照,从肿节风药材图谱中共推断出26个峰的分子量;从清热消炎宁胶囊图谱中推断出16个峰的分子量,共鉴定出5个化合物。结论:液相色谱-质谱联用技术快速分析中药化学组分具有独特优势。Objective： To study and compare the main chemical constituents of Sarcandra glabra and qingrexiaoyanning capsules which were extracted by acetic ether. Methods： The sample solution were analyzed by a Zorbax C18 column with a gradient mobile phase composed of acetonitrile and 0. 1% formic acid solution. Both UV and mass spectrometry detector were used simutaneously, full-scan detection mode was evaluated for the identification of all LC peaks. Results： We analyzed the mass spectrum of every LC peak and identified 26 molecular mass from the ion ehromatogram of Sarcandra glabra extraction and 16 molecular mass from the extractions of qingrexiaoyanning capsule. 5 compounds were identified. Conclusion： High performance liquid chromatography-mass/mass spectrometry has special advantages on analyzing the chemical constituents of traditional Chinese medicine.

关 键 词：肿节风 清热消炎宁胶囊 高效液相色谱-电喷雾串联质谱 

分 类 号：R284.1[医药卫生—中药学]