金针菇多糖对小鼠免疫细胞产生细胞因子及对荷瘤小鼠血清细胞因子含量的影响  

Effect of Flammulina velutipes Polysaccharides on Production of Cytokines by Murine Immunocytes and Serum Levels of Cytokines in Tumor-bearing Mice

作　　者：常花蕾[1] 雷林生[1] 余传林[1] 朱正光[1] 陈娜娜[1] 吴曙光[1] 

机构地区：[1]南方医科大学药学院药物研究所,广东广州510515

出　　处：《中药材》2009年第4期561-563,共3页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金(7005189)

摘　　要：目的:研究金针菇多糖(FVP)对小鼠免疫细胞产生肿瘤坏死因子-α(TNF-α)、干扰素-γ(INF-γ)、白细胞介素-2(IL-2)的影响。方法:MTT比色法检测细胞代谢活力,ELISA方法检测TNF-α、INF-γ、IL-2的含量。结果:FVP(200、100、50μg/mL)可增强小鼠脾细胞及腹腔渗出细胞代谢MTT的活力,促进小鼠脾细胞分泌TNF-α、IFN-γ、IL-2,促进小鼠腹腔渗出细胞分泌TNF-α,并且以促进TNF-α分泌作用最为明显;FVP(100、50、25 mg/kg)可以提高荷S180瘤小鼠血清TNF-α、IFN-γ的含量。结论:FVP可能通过促进小鼠免疫细胞产生TNF-α、IFN-γ、IL-2而发挥免疫调节功能。Objective ：To study the effect of Flammulina velutipes polysaccharides （FVP） on the production of tumor necrosis factorα （TNF-α）,interferonγ（ INF-γ） and interleukin 2 （IL-2） by murine immunocytes. Methods：The cell＇s metabolic activity was determined with methylthiazolyl tetrazolium（MTT） colorimetry assay and the amounts of TNF-α ,INF-γ and IL-2 were measured by ELISA. Results：FVP （200,100,50 μg/mL）could promote the metabolic activity of murine splenocytes and peritoneal exudate cells（PEC） and increase the amounts of TNF-α, INF-γ and IL-2 in the supernatants of splenocyte cultures,and the amount of TNF-α in PEC cultures, with the most marked increase on TNF-α level. FVP（ 100,50,25 mg/kg）could raise the serum levels of TNF-α and INF-γ in S180 tumor-bearing mice. Conclusion：FVP may regulate murine immune function through promoting the production of TNF-α, INF-γ and IL-2.

关 键 词：金针菇 多糖 免疫细胞 S180实体瘤 细胞因子 

分 类 号：R285.5[医药卫生—中药学]