半枝莲提取物对DEN诱发大鼠肝癌的抑制作用  

Inhibitory Effects of Scutellaria barbate Extracts on Diethylnitrosamine-induced Hepatocarcinoma in Rats

作　　者：代志军[1] 王西京[1] 纪宗正[2] 刘小旭[1] 康华峰[1] 刁岩[1] 马小斌[1] 刘蕾[1] 任宏涛[1] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤科,陕西西安710004 [2]西安交通大学医学院第二附属医院普外科,陕西西安710004

出　　处：《中药材》2009年第4期568-571,共4页Journal of Chinese Medicinal Materials

基　　金：陕西省科技攻关项目[2006K16-G5(1)];西安市科技创新支撑计划(YF07175)

摘　　要：目的:探讨半枝莲提取物(Scutellaria barbateextracts,ESB)对二乙基亚硝胺(diethylnitrosamine,DEN)诱发大鼠肝癌形成的抑制作用。方法:采用低剂量DEN间断给药诱发大鼠肝癌模型。60只清洁级雄性SD大鼠随机分为4组:正常对照组,模型组,ESB高、低剂量组,每组15只。实验16周后停止给药,实验第18周处死所有大鼠,观察各组大鼠肝表面癌结节数、大鼠体质量、肝质量、肝/体质量比,比较各组大鼠体质量的改变,检测血清肝功指标丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(γ-GT)、α-L-岩藻糖苷酶(AFU),并进行肝脏病理组织学检查。结果:造模各组大鼠的ALT、AST、TBIL、ALP、γ-GT、AFU均显著升高(P<0.05),ESB治疗可以减轻肝脏损害。与肝癌模型组比较,ESB各剂量组血清各项肝功指标水平显著下降。病理组织学显示,ESB各剂量组肝脏表面癌结节数较肝癌模型组明显减少,且癌细胞分化程度高。结论:ESB对大鼠实验性肝癌形成具有一定的抑制作用,并能改善肝功能的各项指标。Objective：To investigate the inhibitory effects of Scutellaria barbate extracts on diethylnitrosamine-induced hepatocarcinoma in rats. Methods ：Hepatocarcinoma model rats were induced by diethylnitrosamine （DEN）. Sixty SD male rats were randomly divided into 4 groups ： normal control group, hepatoeareinoma model group, ESB of high dose group and ESB of low dose group. All rats were killed in the 18th week, the levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） ,total bilirubin（ TBIL）, alkaline phosphatase（ALP）, γ-glutamyltransferase （T-GT） and alpha-L-fucosidase （AFU） in serum were measured by biochemical examinations ; Hematoxy and eosin （ HE ） methods were used to examine the changes of liver pathology. Results ： The levels of ALT, AST, TBIL, ALP, T-GT, AFU in hepatocarcinoma model group and ESB grnups were higher than that of control group（ P 〈0. 05 ）. ESB could relieve hepatic injures. The levels of liver function indexes in ESB groups were lower than that of model group. Histological examination demonstrated that the number of liver cancer nodus in ESB groups were lower than that of model group. Furthermore,ESB could attenuate the grade of cancer cell diffrentiation. Conclusion ：ESB could inhibit experimental hepatocarcinoma and relieve hepatic injures in rats.

关 键 词：半枝莲 肝癌 二乙基亚硝胺 

分 类 号：R285.5[医药卫生—中药学]