青光安颗粒含药血清对加压后人小梁细胞活性的影响  

Effect of serum containing qingguang'an grains on pressurized human trabecular cells activity

作　　者：彭清华[1] 曾志成[2] 李波[1] 卓业鸿[3] 

机构地区：[1]湖南中医药大学第一附属医院眼科,中国湖南省长沙市410007 [2]湖南中医药大学,中国湖南省长沙市410007 [3]中山大学中山眼科中心,中国广东省广州市510060

出　　处：《国际眼科杂志》2009年第5期839-842,共4页International Eye Science

基　　金：中国湖南省教育厅科研基金重点资助项目(No.06A052);中国湖南省卫生厅科研基金资助项目(No.204084)~~

摘　　要：目的:观察青光安颗粒对体外加压培养人小梁细胞活性的影响,探讨青光安颗粒预防与治疗原发性开角型青光眼术后眼压回升的作用机制。方法:制备2.5倍,5倍,10倍,20倍青光安颗粒含药血清和5倍益脉康含药血清,用不同药物与浓度的含药血清分别作用体外加压培养人小梁细胞12,24,36h,观察细胞形态学的变化和MTT法检测的细胞活性(OD)的变化。结果:加压后小梁细胞的OD值与青光安颗粒的浓度和作用时间均有关系。青光安5倍,10倍,20倍组都能够明显提高细胞OD值,与空白组比较有显著性差异(P<0.01),10倍,20倍组与益脉康组比较有显著性差异(P<0.01),但是青光安10倍组与20倍组之间没有统计学意义;培养24,36h的OD值明显高于培养12h的OD值(P<0.01),但是培养24h与培养36h时间段之间没有统计学意义。结论:青光安颗粒能够提高加压后人眼小梁细胞的活性。AIM： To observe the effects of serum containing qingguang＇an grains on the activity of pressurized human trabecular cells cultured in vitro , and to investigate the mechanism of action in preventing and reducing the as census of postop intraocular pressure on primary open angle glaucoma. METHODS： The qingguang＇an grains-contained serum was prepared,and the pressurized human trabecular cells were incubated with serum containing 2.5 times,5 times, 10 times and 20 times qingguang＇an grains and 5 times yimaikang tablets,respectively,for 12 hours, 24 hours,and 36 hours. The cell morphology was observed and cell activity（OD）was detected by MTT. RESULTS： OD value of pressurized human trabecular cells is concerned with concentration and action time of qingguang＇an grains. On OD value,5 times, 10 times and 20 times qingguang＇an Grains groups were higher than blank group（ P〈 0.01 ） ,10 times and 20 times qingguang＇an grains groups were higher than yimaikang Tablets group（ P 〈 0.01 ）, but no conspicuous different between 5 times qingguang＇an grains groups and 5 times yimaikang Tablets group; OD value of the cells cultured in qingguang＇an grains-contained serum for 24 hours and 36 hours were higher than that cultured for 12 hours（ P〈 0. 01 ）, but no conspicuous different between 24 hours and 36 hours. CONCLUSION. Serum containing qingguang＇an grains can raise pressurized human trabecular cells activity.

关 键 词：青光安颗粒 含药血清 人小梁细胞 活性MTT法 

分 类 号：R285.5[医药卫生—中药学]