小麦幼芽水分胁迫诱导蛋白的特征  

The Characteristics of Induced Protein in Shoots of Wheat Seedlings Under Water Stress

作　　者：李妮亚[1] 高俊凤[1] 汪沛洪[1] 

机构地区：[1]西北农业大学基础科学系,陕西杨陵712100

出　　处：《植物生理学报（0257-4829)》1998年第1期65-71,共7页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金

摘　　要：水分胁迫（－1．2MPaPEG-6000）处理萌动的小麦种子，24h后诱导小麦幼芽产生41．5kD蛋白，其含量随着胁迫时间延长明显增加，48h时含量最高，到72h后不再变化。复水后，该蛋白消失；再胁迫48h时则又出现，其含量与处理24h时相当。41．5kD诱导蛋白主要位于细胞器膜上，细胞质中几乎不存在。41．5kD蛋白主要溶于10％NaCl提取液中，其等电点为pl5．65。该蛋白的氨基酸组成中，脯氨酸含量最高，其次为丙氨酸、天冬氨酸、谷氨酸、甘氨酸，没有发现半胱氨酸和组氨酸。Changs in protein profilesinduced by waer sbess (- 1. 2 MPaPEG) were investigated in shoots of ger-ndnating seeds of wheat. A protein(poforPtide) with an apmpnt MW of41 .5 kD was induced tmder waer sbess(Fig. 1 ). Its content increased with thehme of treatment. The level of 41. 5 kDprotein was enhanced significantly in the48 h of the beatmnt. Afer rewtering,the 41. 5 kD protein disappeared-Howwer, the 41. 5 kD protein appearedagain after rebeatmnt, and the level 0f4l. 5 kD protein was sindlar to tha aftertreatInnt of 24 h. The restilts of scans ofSDS-PAGE of proteins from the shoots ofwheat seediings showed that someoplypeptides disappeared, the content ofothers(e. g 36- 8 kD) increased andsome new polypeptides in the mp of l4to 25 kD were induced tmder watr sbess(Fig- 2). Delindnare results of subcel-lular forhonation indicated that the 41. 5kD protein was located dririly on or-ganelle membranes, almost none in cy-toso(Fig. 3 ). It was present Inainly inexusct with 10% NaCl(Fig.4). Analsisof itS theno acid composition sh0wd thatproline was the most abundant one, withAsp, Gly, Glu and kla in lesser amouns;no Cys and fus were found(Table 1 ).The isoelectric Point of 4l. 5 kD pmteinwas pI 5. 65.

关 键 词：小麦 幼芽 诱导蛋白 水分胁迫 

分 类 号：Q948.112.3[生物学—植物学] S512.1[农业科学—作物学]