一种钙调素结合蛋白对小麦质膜H^+-ATP酶活性的调节  

Regulatory Action of a Calmodulin Bindsng Protein on Activi-ty of the Plasma Membrane H^+ -ATPase in Wheat

作　　者：刘华[1] 凌启阆[1] 向左云[1] 尚克进 

机构地区：[1]南开大学生物化学与分子生物学系,天津300071

出　　处：《植物生理学报（0257-4829)》1998年第1期91-94,共4页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金

摘　　要：CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleoptile elongallon and pbonsecrehon. In oIder to inveshgatewhether BP10 inhibitS Picton secrehonand cell elongaion thidgh inhibitingthc achvity of Plasrna mernbare (PM)H+ -ATPase, the effect of BP-10 on theachvity of wheat coleoptile PM H+ -AT-Pase has been studied. It was observedtha neither IAA nor BP-10 had any effect on the achvity of PM H+ -ATPaSein vitro. In vho, however, the activity0f Al-treated PM H+-ATPaSe washigher than tha of the control, and BP-10 peilicanx iwhbital atinduedachvity by 87- 8% (rig. 1) - In addi-hon, the inhibitory effect cotild be re-versed by IAA after BPIO was removedfrom the mediurn, and it could also beovercome bV the addihon of CaM.Therefore, it appears that BP-10 in-hibits cell responses to andn by affect-ing the achvity of PM H+ -ATPase andthereby inhibiting Proton secrehon. As aresult, cell elongaon is inhibited. AndCaM is also involved in this pmeess-CaM BP-10 (BP-10) is anovel endogenous calmodalin (CaM )-binding Protein tha was observed in andisolated from Plant. In our prelidrimpstudies it has been shown tha BP-10specifically inhibits adrin (IAA)-in-duced coleoptile elongallon and pbonsecrehon. In oIder to inveshgatewhether BP10 inhibitS Picton secrehonand cell elongaion thidgh inhibitingthc achvity of Plasrna mernbare (PM)H+ -ATPase, the effect of BP-10 on theachvity of wheat coleoptile PM H+ -AT-Pase has been studied. It was observedtha neither IAA nor BP-10 had any effect on the achvity of PM H+ -ATPaSein vitro. In vho, however, the activity0f Al-treated PM H+-ATPaSe washigher than tha of the control, and BP-10 peilicanx iwhbital atinduedachvity by 87- 8% (rig. 1) - In addi-hon, the inhibitory effect cotild be re-versed by IAA after BPIO was removedfrom the mediurn, and it could also beovercome bV the addihon of CaM.Therefore, it appears that BP-10 in-hibits cell responses to andn by affect-ing the achvity of PM H+ -ATPase andthereby inhibiting Proton secrehon. As aresult, cell elongaon is inhibited. AndCaM is also involved in this pmeess-

关 键 词：钙调素 结合蛋白 生长素 小麦 ATP酶 质膜 

分 类 号：Q946.5[生物学—植物学]