阿是止泻胶囊对甲亢性腹泻大鼠十二指肠黏膜肥大细胞的影响  

Effect of Asi-antidiarrheal capsule on mast cell in duodenum mucosa of thyroid hormone-induced diarrheic rats

作　　者：毕长龙[1] 张瑛琦[1] 翟爱霞[2] 成志锋[1] 

机构地区：[1]哈尔滨医科大学第四临床医学院内分泌科,黑龙江哈尔滨150001 [2]哈尔滨医科大学微生物学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第2期150-153,共4页Journal of Harbin Medical University

基　　金：黑龙江省科委"十五"攻关基金资助项目(GCOIC138201)

摘　　要：目的探讨阿是止泻胶囊对甲状腺功能亢进(甲亢)性腹泻大鼠十二指肠黏膜肥大细胞(MC)的影响。方法制备甲亢性腹泻大鼠模型。运用阿是止泻胶囊低、中、高剂量及黄连素对其干预,采用免疫组化技术测定对照组、模型组、黄连素组、阿是止泻胶囊低、中、高剂量组大鼠十二指肠黏膜MC数量、脱颗粒比率、面积、周长、等效直径及圆形度。结果①与对照组比较,模型组十二指肠黏膜MC数目、面积、周长、等效直径增大,圆形度变小(P<0.01或P<0.05);②与模型组比较,黄连素组、高剂量组十二指肠黏膜MC数目、面积、周长、等效直径变小,圆形度增大(P<0.01或P<0.05)。结论阿是止泻胶囊能降低十二指肠黏膜MC数目,可能是其治疗甲亢腹泻的机制之一。Objective To investigate the Asi-antidiarrheal capsule＇ s effect on mast cell （MC） in duodenum mucosa of thyroid hormone-induced diarrheic （ hyperthyroid diarrhea, HD） rats. Methods The model of thyroid hormone-induced diarrheic rats was prepared and interfered by low, medium and high dose of Asi-antidiarrheal capsule and berberine. Immunohistochemistry was used to detect the population, ratio of degranulation, area, perimeter, equivalent diameter and cyclo-dimension of the MC in duodenum mucosa of control group （CG） , model group （ MG ） , berberine group （BG） and Asi- antidiarrheal capsule group （AG）. Results Compared with CG, the population, area, perimeter and equivalent diameter of the MC in duodenum mucosa were increased and the cyclo-dimension was decreased in MG （P 〈0.01 or P 〈0.05）. Compared with MG, the population, area, perimeter and equivalent diameter of the MC in duodenum mucosa were decreased and the cyclo-dimension was increased in BG and high dose AG （ P 〈 0.01 or P 〈 0.05 ）. Conclusion Asi-antidiarrheal capsule can decrease the number of MC in duodenum mucosa and that may have potential effect on hyperthyroidism diarrhea.

关 键 词：甲状腺功能亢进 腹泻 阿是止泻胶囊 肥大细胞 

分 类 号：R581.1[医药卫生—内分泌]