肾透明细胞癌患者血清中内皮抑素含量检测及其临床意义  

Clinical significance of serum levels of endostatin in patients with clear cell renal cell carcinoma

作　　者：管考鹏[1] 肖振东[1] 王栋[1] 石泓哲[1] 关有彦[1] 鲁力[1] 韩苏军[1] 李长岭[1] 

机构地区：[1]中国医学科学院肿瘤医院泌尿外科,北京100021

出　　处：《中华外科杂志》2009年第10期737-739,共3页Chinese Journal of Surgery

摘　　要：目的检测肾透明细胞癌患者术前血清中内皮抑素含量，分析其与肿瘤分级、分期的关系。方法2004年3月至2008年10月接受手术治疗的肾透明细胞癌患者138例，其中男性102例，女性36例，平均年龄63．2岁；T1期73例，12期39例，13期20例，T4期6例。40例性别、年龄相应的健康人作为对照组，应用双抗体夹心ELISA法检测其术前血清中内皮抑素水平。结果患者术前血清中内皮抑素含量均值为93．1μg／L，与健康对照组（78．9μg／L）差异无统计学意义（P〉0．05）。T1期患者术前血清内皮抑素水平为80．4μg／L，低于r12期（107．5μg／L，P〈0．01）、T3期（102．7μg／L，P〈0．05）及T4期（120．7μg,／L，P〈0．01），而后三组之间差异无统计学意义（P〉0．05）。T1期患者与对照组无明显差异（P〉0．05）。转移组患者术前血清内皮抑素含量为118．4μg／L，高于未转移组的89．5μg／L（P〈0．05）；单纯淋巴结转移组及远处转移组患者之间差异无统计学意义。G3-4级患者血清内皮抑素水平为111．8μg／L，高于G1级（80．4μg／L）和G2级（86．2μg／L）（P〈0．01）。结论肾透明细胞癌患者术前血清内皮抑素含量与肿瘤的高分级、高分期相关，可能有助于患者预后及肿瘤分化程度判断。Objective To assess serum levels of endogenous endostatin in patients with clear cell renal cell carcinoma （CCRCC） and to determine the relationship of these levels to tumor stage, grade. Methods From March 2004 to October 2008, preoperative serum were obtained from 138 consecutive patients with CCRCC （73 patients in T1,39 patients in T2, 20 patients in T3, and 6 patients in T4） and 40 healthy controls. Serum levels of endostatin were measured by sandwich-ELISA. Associations between circulating endostatin levels and clinicopathologic factors and clinical outcome were determined. Results Endostatin levels did not differ significantly between the patients with CCRCC （93.1 μg/L） and healthy controls （78.9 μg/L, P 〉0. 05）. Serum levels of endostatin were significantly higher in the T2-4 CCRCC patients （ 107. 2 μg/L） than those of the T1 patients （ 80. 4 μg/L, P 〈 0. 01 ）. No significant difference was found in the endostatin levels among the T2-4 patients, or between healthy controls and the T1 patients. The serum endostatin concentration was significantly higher in the metastasis group （118.4 μg/L） than in the no metastasis group （89. 5 μ/L, P 〈 0.05 ）, but there was no significant difference between patients with distant metastasis group （ 122. 0 μg/L） and lymph nodes metastasis （ 110. 0 μg/L, P 〉 0. 05 ）. Patients with G3-4 tumors had significantly higher endostatin levels （ 111.8 μg/L） than those of patients with G1 （80. 4 μg/L） and G2 tumors （86. 2 μg/L, P 〈 0. 01 ）, but endostatin levels did not differ significantly between the two groups （P 〉 0. 05）. Conclusion Preoperative serum levels of endostatin elevated in patients with CCRCC and associated with higher stage and grade.

关 键 词：肾肿瘤 内皮抑素类 酶联免疫吸附测定 新生血管化 病理性 

分 类 号：R686[医药卫生—骨科学]