口腔鳞状细胞癌组织乏氧状况的初步研究  

A pilot study on hypoxia of squamous cell carcinoma in oral cavity

作　　者：刘文曙[1] 尚建伟[2] 高岩[2] 郭传瑸[1] 

机构地区：[1]北京大学口腔医学院·口腔医院口腔颌面外科,100081 [2]北京大学口腔医学院·口腔医院病理科

出　　处：《中华口腔医学杂志》2009年第5期282-285,共4页Chinese Journal of Stomatology

摘　　要：目的 检测口腔鳞状细胞癌组织乏氧程度，探讨口腔鳞状细胞癌组织乏氧影响因素，认识和利用肿瘤乏氧区域的特性，为治疗提供依据。方法经临床和病理证实的口腔鳞状细胞癌组织石蜡标本42例，建立口腔鳞状细胞癌动物移植瘤模型，采用单光子发射计算机断层成像（single photon emission computerized tomography，SPECT）乏氧显像技术检测癌组织的乏氧程度；采用免疫组织化学方法检测肿瘤乏氧标志物（hypoxiainduciblefactor1α HIF—1α）在口腔鳞状细胞癌组织中的分布，并分析与临床病理资料的相关性。结果乏氧显像剂在动物模型鳞状细胞癌组织内高浓度聚集，与肿瘤体积呈线性相关，相关指数为0．926（P〈0．05），呈全层乏氧；口腔鳞状细胞癌组织中HIF—1α蛋白高表达，且在低分化、淋巴结转移及高临床分期组中的表达较高分化、无淋巴结转移及低临床分期组高（P〈0．05）；正常口腔黏膜组织中HIF—1α仅无表达。结论口腔鳞状细胞癌组织乏氧区域分布广泛。HIF-1α在口腔鳞状细胞癌组织中高表达，可能在口腔鳞状细胞癌的发生和发展中起重要作用。Objective To examine the extent of hypoxia in oral squamous cell carcinoma and investigate the factors related to the hypoxia. Methods An animal model of oral squamous cell carcinoma was established and single photon emission computed tomography （SPECT） with 99mTc-HL91 used to detect the hypoxia extent of the oral squamous cell carcinoma. The expression of hypoxia inducible factor 1 alpha （HIF-1α） was examined by immunohistochemical staining in 42 cases of formalin-fixed paraffin-embed squamous cell carcinoma. Results The uptake of 99mTc-HLgl in the tumor tissue was higher than that in normal tissue and had linear relation with the tumor size （ P 〈 0.05 ）. There was no HIF-1α expression in the normal oral mucosa. The expression of HIF-1α was high in oral mucosa carcinoma and closely related to the differentiation degree of tumor and metastasis of lymph node （ P 〈 0. 05 ） . Conclusions Tumor tissue had broad hypoxic region. HIF-1α highly expressed in oral squamous cell carcinoma and may play an important role in carcinogenesis and aggression.

关 键 词：癌 鳞状细胞 体层摄影术 发射型计算机 单光子 肿瘤治疗方案 

分 类 号：R686[医药卫生—骨科学]