细胞角蛋白18-mRNA的表达与口腔鳞状细胞癌侵袭转移的关系  

Correlation between the expression of cytokeratin-18-mRNA and invasion and metastasis of oral squamous cell carcinoma

作　　者：舒萍[1] 赵德安[2] 鲁大鹏[1] 

机构地区：[1]首都医科大学口腔医学院急诊科,100050 [2]福建医科大学附属厦门市第一医院头颈外科

出　　处：《中华口腔医学杂志》2009年第5期286-288,共3页Chinese Journal of Stomatology

基　　金：北京市留学人员科技活动择优资助项目（2003）;北京市卫生局留学回国人员科研专项经费（2003）;教育部留学回国人员科研启动基金（教外司留2004176号）

摘　　要：目的 探讨单层细胞角蛋白（cytokeratin，CK）18-mRNA表达与口腔鳞状细胞癌（oralsquamous cell carcinoma，OSCC）的局部侵袭与血道转移的关系。方法选用23例OSCC组织及相应癌旁组织，通过半定量反转录聚合酶链反应（RT—PCR）方法在不同时段检测CK-18-mRNA在OSCC外周血中的表达，探讨CK-18-mRNA与OSCC远处转移及复发的关系。结果23例OSCC组织中有16例CK-18-mRNA呈阳性表达，但表达量存在明显差异（P〈0．01），与肿瘤部位无关。OSCC组织中CK-18-mRNA表达量[（29．30±22．37）％]显著高于癌旁组织[（15．50±11．07）％，P〈0．01]；其表达量与临床分期、分化程度及淋巴结转移有关。23例OSCC术前外周血中有4例出现CK-18-mRNA表达，仅1例在随访过程中始终存在CK-18-mRNA的表达。结论CK-18-mRNA可作为预测OSCC侵袭转移的辅助指标，中晚期患者外周血中CK-18的表达不能作为确诊OSCC远处转移的指标，但通过连续观察能够为监测OSCC转移与复发提供参考依据。Objective To investigate the relationship between the expression of cytokeratin （CK）- 18 and biological behavior of oral squamous cell carcinoma （OSCC）. Methods Twenty-three patients with OSCC were investigated for the expression of CK-18-mRNA by semi-quantitative reverse transcriptasepolymerase chain reaction （RT-PCR）. Correlations between clinical stages, pathological differentiation, lymphatic metastasis and the expression of CK-18-mRNA were evaluated. CK-18-mRNA expression of peripheral blood from the 23 patients and 23 healthy people were also examined. During follow-up after operation, the peripheral blood was collected again for the expression of CK-19-mRNA. Results Expression of CK-18-mRNA was found in 16 patients. The expression of CK-18-mRNA was significantly associated with clinical stages, tumor differentiation and lymphatic metastasis. CK-18-mRNA was positive in 4 of 23 blood specimens before operation, but during follow-up only 1 of 23 patients was still positive in peripheral blood. Conclusions CK-18 may provide additional information in forecasting the metastasis of OSCC and serve as a reference in monitoring recurrence.

关 键 词：癌 鳞状细胞 角蛋白18 肿瘤转移 

分 类 号：R686[医药卫生—骨科学]