纳米羟基磷灰石的制备及其对牙本质小管封闭效果的初步观察  

The preparation of nano-hydroxyapatite and preliminary observation on its effects on the occlusion of dentinal tubules

作　　者：王浙君[1] 撒悦[1] 马霄[1] 王贻宁[2] 蒋滔[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,430079 [2]武汉大学口腔医学院修复科

出　　处：《中华口腔医学杂志》2009年第5期297-300,共4页Chinese Journal of Stomatology

基　　金：湖北省科技攻关计划项目（2007AA301B28）

摘　　要：目的 通过合成纳米羟基磷灰石（hydroxyapatite，HA）以及体外实验观察其对牙本质小管的封闭效果，为纳米HA的临床应用提供依据。方法采用磷酸氢二铵[（NH4）2HPO4]和硝酸钙[Ca（NO3）2]合成纳米HA，扫描电子显微镜（scanning electron microscope，SEM）、透射电子显微镜（transmission electron microscope，TEM）、傅里叶变换红外光谱计（Fourier transform infrared spectrometer，FTIR）和X射线衍射仪（X—ray diffractometer，XRD）检测纳米HA。选取8颗健康第三磨牙制备24片牙本质薄片，按随机数字表分为酪蛋白磷酸肽钙磷复合体（casein phosphopeptideamorphous calciumphosphate，CPP—ACP）组、纳米HA组及空白对照组，每组8片；空白对照组不做处理，CPP—ACP组和纳米HA组每天分别用CPP-ACP和纳米HA处理牙本质薄片2次，7d后SEM观察牙本质表面。结果经SEM、TEM、FTIR和XRD检测，证实本项实验制备的HA为纳米HA；SEM观察纳米HA对牙本质小管的封闭效果明显高于CPP—ACP组和空白对照组。结论在体外条件下，与CPP—ACP相比，纳米HA可更有效地封闭暴露的牙本质小管。Objective To synthesize nano-hydroxyapatite （nano-HA） and evaluate its effect on the occlusion of dentinal tubules in vitro, and therefore provide evidence for the clinical application. Methods （NH4）2HPO4 and Ca（NO3）2 were used to form nano-HA, which was characterized by scanning electron microscopy（ SEM ）, transmission electron microscopy （TEM）, Fourier transform infrared spectrometer （FTIR） and X-ray diffractometer（XRD） respectively. Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups ,which were control group, casein phosphopetide-amorphous calcium phosphate（CPP-ACP） group, and nano-HA group. After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days, the surface of slab dentin was observed using SEM. Results SEM, TEM, FTIR, and XRD tests showed that nano-HA was synthesized successfully. SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group. Conclusions In comparison with CPP-ACP, nano-HA could occlude dentinal tubules more effectively in vitro.

关 键 词：羟基磷灰石类 牙本质敏感 酪蛋白磷酸肽钙磷复合体 

分 类 号：R686[医药卫生—骨科学]