DNA跨损伤修复基因在人脑胶质瘤中的表达  

Expression study of DNA translesion synthesis genes in hnman primary glioma

作　　者：奚才华[1] 胡锦[1] 王慧博[2] 张舒羽[3] 卢大儒[3] 

机构地区：[1]上海交通大学附属第六人民医院急性创伤急救中心,200233 [2]哈尔滨医科大学第一附属医院神经外科 [3]复旦大学生命科学院遗传学研究所遗传工程国家重点实验室

出　　处：《中华医学杂志》2009年第19期1309-1312,共4页National Medical Journal of China

基　　金：国家自然科学基金（30371459）

摘　　要：目的研究DNA跨损伤修复基因Revl、Polξ（Rev3和Rev7）、Polη、Polι、和Polk在脑胶质瘤中的表达，探讨这些基因的表达水平与脑胶质瘤发生、发展的关系。方法利用SYBRGreen实时定量PCR技术检测DNA跨损伤修复基因在85例原发脑胶质瘤病人和14名正常脑组织中的mRNA表达情况。结果与正常脑组织相比，Revl基因在Ⅳ级胶质瘤中表达上调（△Ct为5．55±1．12，P〈0．05）；Rev3基因在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤中表达上调（△Ct分别为5．46±1．35、5．13±1．53和6．14±1．10，均P〈0．05）；Rev7基因在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤中表达上调（△Ct分别为5．88±0．82、5．41±1．43和5．56±0．96，均P〈0．05）；Polη基因在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤中表达上调（△Ct分别为5．97±1．15、5．83±1．03和6．34±1．0l，均P〈0．05）；Polι基因在Ⅱ～Ⅳ级胶质瘤中表达上调（ACt分别为6．67±1．17、6．33±1．39和7．03±1．06，均P〈0．05）；PolK基因在Ⅳ级胶质瘤中表达上调（△Ct为5．25±1．58，P〈0．05）。Spearman秩相关分析表明，跨损伤修复基因Revl、Rev3、Polm、PolL和PolK的表达量与肿瘤病理学分级呈正相关（均P〈0．01）。结论DNA跨损伤修复基因可能与胶质瘤的发生、进展相关。Objective To detect the expressions of Revl, Polξ, Polη, Pol ι and Pol K mRNA in human primary glioma tissues, and to analyze the relationship between the expression and the pathological grade. Methods We applied SYBR Green real-time PCR to investigate the mRNA expressions of the 6 genes in 85 primary glioma tissues and 14 normal brain tissues. Results The expressions of 4 genes including Rev3, RevT, Pol η and Pol ι were significantly （ P 〈 0. 05 ） higher in glioma tissues in grade Ⅱ , Ⅲ and Ⅳ as compared to normal brain tissues. The expressions of Revl and Pol K were significantly （P 〈 0. 05 ） higher in grade IV glioma compared to the normal brain tissue. The positive correlation existed （ P 〈 0. 01 ） between the expression of all genes mRNA and the pathologic grade of the human glioma, except for Rev7. Conclusion The up-regulation of DNA translesion synthesis genes, including Revl, Rev3, Rev7, Pol η, Pol ι and Pol K, may be associated with pathogenesis of glioma.

关 键 词：DNA跨损伤修复基因 人脑胶质瘤 治疗 临床 

分 类 号：R686[医药卫生—骨科学]