神经调节素对猕猴脑缺血再灌注损伤的干预作用  

Interfering effect of neuregulin upon cerebral ischemic reperfusion injury in rhesus monkeys

作　　者：张睿[1] 李琴[1] 王涛[1] 杜芳[1] 郭云良[1] 

机构地区：[1]青岛大学医学院附属医院脑血管病研究所,266003

出　　处：《中华医学杂志》2009年第19期1361-1364,共4页National Medical Journal of China

基　　金：山东省自然科学基金项目（Z2007D05）;青岛市科技局基金项目（06-2-2-4-NSH-1）

摘　　要：目的探讨神经调节素-1β（NRG-1β）对猕猴脑缺血再灌注损伤的保护作用。方法成年健康猕猴12只，经股动脉介入手术，将微球囊导管插入大脑中动脉阻断其血流，建立大脑中动脉缺血再灌注（MCAO／R）模型。治疗组动物于缺血2h再灌注前经微球囊导管注射NRG—1β，对照组同步给予等量的生理盐水，然后退出微球囊导管实现再灌注22h。应用Task—oriented评分评价动物的神经行为功能、氯化三苯基四氮唑（TTC）染色和磁共振成像（MRI）检测脑梗死体积的变化。结果对照组动物脑缺血2h即出现神经功能缺损表现，再灌注22h（60-4-16）未见显著性变化；NFG-1β治疗后（61±16）未能明显改善猕猴神经功能（P〉0．05）。对照组动物脑缺血2h在MRI弥散加权成像（DWI）即出现高信号区，但T1、T2相表现不明显；再灌注22h在相应部位出现典型的T1低信号、T2高信号区，DWI高信号增强，TTC染色显示明显的缺血病灶。NFG-1β组T1、T2和DWI的异常信号区和TTC脑梗死体积未见明显减小（P〉0．05）。结论NRG-1β对脑缺血再灌注损伤的保护作用有待于进一步研究。Objective To study the interfering effect of neuregnhn-1β（NRG-1β）on the cerebral ischemic reperfusion injury in monkeys. Methods The models with middle cerebral artery occlusion reperfusion （MCAO/R） were established by inserting a micro-balloon catheter intra-arterially into MCA from femoral artery in 12 adult healthy monkeys. The NRG-1βwas injected into MCA from micro-balloon catheter in the animals of treatment group at 2 h post-ischemia while the normal saline was simultaneously injected in the animals of control group. Then the micro-balloon catheter was withdrawn from the MCA to perfuse for 22 h. The nervous behavioral function was evaluated by task-oriented score and the infarct volume measured by TIC and MRI. Results The animals of control group had an onset of nervous functional disorders at 2 h post-ischemia and had no significant difference at reperfusion 22 h （60 ＋ 16 ） ; the nervous function of monkeys had no improvement after NFG-1β treatment （61±16） （P 〉0.05）. The diffuse weight imaging （DWI） showed a high signal area but T1 and T2 imaging had no significant changes at 2 h post-ischemia in control group animals; while the T1 and T2 imaging showed a typical high signal areas, DWI high signal area expanded and TIC staining ischemic area appeared at reperfusion 22 h. The T1, T2 and DWI abnormal signal areas and TFC cerebral infarct volume did not shrink significantly in NFG-1β treatment group （ P 〉 0. 05）. Conclusion The neuroprotective effects of NRG-1β upon cerebral ischemic reperfusion injury needs to be further studied.

关 键 词：神经调节素 脑缺血 再灌注 弥散加权成像 猕猴 

分 类 号：R686[医药卫生—骨科学]