子宫内膜腺癌组织中miRNA的差异表达  

Expression of microRNAs in endometrioid adenocarcinoma

作　　者：刘霞[1] 夏伟[2] 代荫梅[1] 邵宁生[2] 张为远[1] 

机构地区：[1]首都医科大学附属北京妇产医院,100026 [2]军事医学科学院

出　　处：《中华医学杂志》2009年第19期1365-1367,共3页National Medical Journal of China

基　　金：国家自然科学基金（30772322）

摘　　要：目的探讨miRNA在子宫内膜腺癌及其癌旁组织中的表达差异。方法收集子宫内膜腺癌患者癌灶和癌旁内膜组织，分别采用Trizol一步法提取总RNA，利用含有miRNA探针的芯片检测系统进行miRNA表达谱差异分析。结果子宫内膜腺癌与癌旁组织中111个miRNA表达存在显著差异，其中腺癌组织中68个miRNA表达上调，43个表达下调。结论miRNA可能参与子宫内膜腺癌的发生发展过程，这为进一步揭示子宫内膜腺癌发生的分子机制提供了依据。Objective To analyze miRNA expression profile of endometrioid adenocarcinoma. Methods Collect Endometrium was collected from cancer and paracancer tissue. And the total RNA was extracted. The microarray detection system was employed to analyze whether there was difference in microRNA expression between cancer tissue and paracancer tissue. Results A total of 111 differentially expressed miRNA were found, including 68 over-expression miRNA and 43 low- expression. Conclusion MiRNA may play important roles in tumorigenesis of endometrioid adenocarcinoma. The study of miRNA contributes to elucidate the molecular mechanism of endometrial cancer.

关 键 词：子宫内膜腺癌 MIRNA MIRNA芯片 

分 类 号：R686[医药卫生—骨科学]