粘细菌Sorangium cellulosum So0087-3-3菌体提取物的分离和鉴定  

Isolation and Elucidation Metabolites from Mycelial Culture of Sorangium cellulosum So0087-3-3

作　　者：鲁春华[1] 赵林[2] 李越中[2] 沈月毛[1] 

机构地区：[1]厦门大学生命科学学院 福建省药物工程实验室,福建厦门361005 [2]山东大学粘细菌及医药微生物技术研究室,山东济南250100

出　　处：《厦门大学学报（自然科学版）》2009年第3期414-417,共4页Journal of Xiamen University：Natural Science

基　　金：国家科技部“863”目标导向课题(20060102Z1083)资助

摘　　要：以福建莆田地区分离得到的粘细菌Sorangium cellulosum So0087—3—3为研究对象，通过发酵培养对菌体的化学成分进行研究．从5L发酵菌体提取物中分离得到7个化合物，通过1D，2DNMR以及质谱数据鉴定了化合物1～3的结构，分别为2-（4-hydroxyphenyl）ethylamine，麦角甾醇，过氧麦角甾醇；初步确定化合物4～7为脂肪酸甘油酯类化合物．Myxobacteria are Gram-negative gliding bacteria that noted for complicated multicellular social behavior and the excellent ability to produce various bioactive compounds. Recently, we have embarked on a research program looking for new structural and bioactive metabolites from myxobacteria, and the strain So0087-3-3 was isolated from the soil of Putian, Fujian, and was identified as Sorangium cellulosum. Herein,we reported the fermentation,isolation and structure elucidation of the compounds from this strain. 5 L cultures were fermented and the chemical constituent from mycelia culture was studied. Seven compounds were isolated and purified by column chromatography,and the structures of compounds 1 -3 were elucidated respectively as 2-（4-hydroxyphenyl）ethylamine, ergosterol, peroxy-ergosterol by spectroscopic analyses, including 1D-, 2D-NMR data and mass-spectrometric analyses. Compounds 4 -7 were identified as glyceride.

关 键 词：Sorangiumcellulosum So0087-3-3 NMR 甾醇 脂肪酸甘油酯类化合物 

分 类 号：R93[医药卫生—生药学]