疏叶骆驼刺茎段离体快繁技术的研究  

Study on in vitro propagation of Alhagi sparsifolia cuttings

作　　者：赵洁[1] 张博[1] 王玉祥 贾广云[1] 李培英[1] 

机构地区：[1]新疆草地资源与生态重点实验室新疆农业大学草业与环境科学学院,新疆乌鲁木齐830052

出　　处：《草业科学》2009年第5期72-75,共4页Pratacultural Science

基　　金：新疆维吾尔自治区高技术研究发展计划项目(200711101)

摘　　要：以疏叶骆驼刺Alhagi sparsifolia无菌苗茎段为外植体,探讨了不同浓度6-苄氨基嘌呤(6-BA)和萘乙酸(NAA)、水解糖蛋白(LH)、赤霉素(GA3)在疏叶骆驼刺茎段离体快繁中的作用以及NAA在生根时的作用。结果表明:1)当NAA浓度为0.1 mg/L时,降低6-BA浓度,有利于芽的生长,反之,有利于丛生芽分化;2)LH能促进苗的生长,增加芽分化能力,MS+0.5 mg/L 6-BA+0.1 mg/L NAA+1.5 g/L LH为疏叶骆驼刺茎段快繁最佳培养基,增殖倍数为6.8;3)GA3对骆驼刺茎段离体培养无显著促进作用;4)1/2MS+0.1 mg/L NAA为最佳生根培养基,生根率为71.7%。Using Alhagi sparsifolia cuttings as explants, the effects of different concentration of 6-BA and NAA, LH, GA3 on in vitro propagation and the effects of NAA on seedling rooting were discussed. The results are as follows： 1） with 0.1 mg/L NAA, reducing 6-BA concentration was benefit to stem growth. On the contrary, it is benefit to differentiation of buds;2） LH is advantaged to seedling growth and differentiation of buds,MS＋0.5 mg/L 6-BA ＋ 0.1 mg/L NAA ＋1.5 g/L LH was the better induction medium. Propagation muhiple was 6.8 ;3）The effect of GA3 on in vitro propagation of A. sparsifolia was not significant;4）1/2MS＋ 0.1 mg/L NAA was the best medium for roo- ting, the rooting rate was 71.7%.

关 键 词：疏叶骆驼刺 茎段 离体快繁 

分 类 号：S54[农业科学—作物学]