苜蓿草粉对禾花鲤蛋白酶和淀粉酶活性影响的研究  

Effects of alfalfa meal on protease and amylase activities of common carps

作　　者：张春梅[1] 施传信[1] 王成章[2] 何云[3] 刘圈炜[2] 

机构地区：[1]商丘师范学院生命科学系,河南商丘476000 [2]河南农业大学牧医工程学院,河南郑州450002 [3]河南科技学院动物科学学院,河南新乡453003

出　　处：《草业科学》2009年第5期128-134,共7页Pratacultural Science

基　　金：国家农业科技成果转化资金项目;河南省科技厅重大科技攻关项目(0422010300)

摘　　要：选取来源一致、规格整齐的2龄禾花鲤1 200尾,采用单因子完全随机设计,分为5个处理,每处理3个重复,每重复80尾鱼,分置于15个试验池中。各处理苜蓿Medicago sativa草粉的添加量分别为0(对照组)、40(试验Ⅰ组)、80(试验Ⅱ组)、120(试验Ⅲ组)和160 g/kg(试验Ⅳ组),正试期50 d,观测苜蓿草粉对禾花鲤前、中、后肠及肝胰脏蛋白酶和淀粉酶活性的影响。结果表明:1)添加苜蓿草粉对前肠蛋白酶活性有显著影响(P<0.05),其中试验II组显著高于对照组和试验Ⅳ组,但和试验Ⅰ、Ⅲ组差异不显著(P>0.05)。添加苜蓿草粉对中、后肠及肝胰脏蛋白酶活性均无显著影响(P>0.05),但它们有相同的变化趋势,即试验Ⅰ、Ⅱ、Ⅲ组鱼的蛋白酶活性均高于对照组。2)鲤鱼的前肠、中肠、后肠蛋白酶活性依次减弱,而肝胰脏的蛋白酶活性远低于肠道。3)添加苜蓿草粉后前肠及中肠淀粉酶活性比较,试验II组显著高于对照组(P<0.05),其余各组与对照组差异不显著(P>0.05),后肠淀粉酶活性与对照组无显著差异(P>0.05)。添加苜蓿草粉能提高鲤鱼肝胰脏淀粉酶活性,其中试验Ⅱ组显著高于对照组(P<0.05),其余试验组与对照组无显著差异(P>0.05)。4)禾花鲤前、中、后肠及肝胰脏4部分淀粉酶的活性有较大的不同,肝胰脏>后肠>中肠>前肠。An experiment was conducted using 1 200 carps to study the effects of alfalfa meal on the activity of digestive enzymes. Carps were randomly divided into five treatments according to a random factorial arrangement, each treatment represented by three replicates of 80 carps each. Carps were fed with diet containing 0（control）, 40, 80, 120 and 160 g/kg （group 1,2,3,4, respectively ）alfalfa meal during the period of 50 days,and activity of protease and amylase in carp＇s foregut, midgut, hindgut and hepatopancreas were investigated. The results showed that： 1） Protease activity in foregut of carps fed with 80 g/kg alfalfa meal was significantly enhanced compared to the control and group 4 （P〈0.05）, but there was no significant difference between group 1 and group 3 （P〉0.05）. Protease activity in midgut, hindgut and hepatopancreas of carps had no significant difference compared to the control （P〉0.05）, but they had the same trend： the activity of protease of group 1, 2 and 3 were higher than the control group. 2）Activity of protease in carp＇s foregut, midgut and hindgut were gradually lowered, And activity of protease in hepatopancreas were far lower than that in guts. 3） Activity of amylase in foregut and midgut of carps fed with 80 g/kg alfalfa meal were significantly enhanced （P〈0. 05）. There were no significant difference between the other groups and the control （P〉 0.05）. Amylase activity in hindgut had no significant difference compared to the control （P〉0.05）. But alfalfa meal enhanced the activity of amylase in hepatopancreas, group 2 was significantly higher than control （P〈0.05）. The other groups had no significant difference compared to the control （P〉 0.05）. 4） There was great difference between activity of amylase in foregut, midgut, hindgut and hepatopanereas. Among the four parts, the activity of amylase ranged from high to low： hepatopancrac〉 hindgut〉 midgut〉 foregut. In summary, appropriate alfalfa meal significantly impro

关 键 词：苜蓿草粉 禾花鲤 蛋白酶 淀粉酶 

分 类 号：S963[农业科学—水产养殖]