HPLC-DAD法测定板蓝根药材及其制剂中主要抗病毒生物碱含量  

Determination of main antiviral alkaloidal constituent in Radix Isatidis and its preparation by HPLC-DAD

作　　者：安益强[1,2] 贾晓斌[2,3] 陈彦[2,3] 孙忠文[4] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏省中医药研究院中药新型给药系统重点实验室,江苏南京210028 [3]国家中医药管理局名医名方适宜剂型重点研究室,江苏南京210028 [4]南京中医药大学药学院,江苏南京210046

出　　处：《中成药》2009年第5期733-736,共4页Chinese Traditional Patent Medicine

基　　金：江苏省中医药领军人才资助项目(2006);苏州市科技计划(SSY0606)

摘　　要：目的:利用HPLC-DAD测定板蓝根药材及其制剂中表告依春(Epigoitrin)和2,4(1H,3H)喹唑二酮(2,4(1H,3H)-quinazolinedione)的含量。方法:ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-(0.1%磷酸+0.03%三乙胺)水溶液=12∶88;流速为0.7 mL/min;检测波长为224 nm,柱温为30℃。结果:表告依春在0.040 9μg^1.103 8μg,2,4(1H,3H)喹唑二酮在0.010 6μg^0.169 6μg与峰面积呈良好的线性关系,回收率分别为98.44%,RSD为1.43%;98.67%,RSD为1.65%。结论:本方法能够很好地测定板蓝根药材及其制剂中主要抗病毒类生物碱表告依春、2,4(1H,3H)喹唑二酮的含量,可用于板蓝根药材及其制剂的质量控制。AIM ： To determine epigoitrin and 2,4 （ 1 H, 3 H） -quinazolinedione in Radix Isatidis and its prepara- tion by HPLC-DAD. METHODS： HPLC was carried out with an Agilent 1200, equipped with ZORBAX SB-C18 column（4.6 mm ×250 mm,5μm）. The mobile phase of aeetonitrile： （0.1% phosphoric acid ＋0.03% triethylamine） aqueous solution = 12： 88. The flow rate was 0.7 mL/min. The detection wavelength was set at 224 nm. The column temperature was at 30 ℃. RESULTS： The linear ranges of epigoitrin was 0.040 9 μg - 1. 103 8 μg; 2,4 （ 1 H,3H ） -quinazolinedione was 0. 010 6 μg - 0. 169 6μg, The recoveries of epigoitrin and 2,4 （ 1 H, 3 H ） - quinazolinedione were 98.44% （ n = 9 ）, with RSD of 1.43% and 98.67%, with RSD of 1.65%, respectively. CONCLUSION ： The method is simple, sensitive and reliable. It can be used for quantitative determination of Radix Isatidis and its preparation.

关 键 词：板蓝根 制剂 表告依春 2 4(1H 3H)喹唑二酮 HPLC-DAD 

分 类 号：R927.2[医药卫生—药学]