HPLC测定刺五加脑灵液中异嗪皮啶和五味子醇甲的含量  

Simultaneous determination of isofraxidin and schisandrin in Ciwujia Naoling (Oral) Liquid by HPLC

作　　者：肖望书[1] 赵陆华[1] 宿婷[1] 

机构地区：[1]中国药科大学分析测试中心,江苏南京210009

出　　处：《中成药》2009年第5期737-739,共3页Chinese Traditional Patent Medicine

摘　　要：目的：建立一种反相高效液相色谱方法,同时测定刺五加脑灵液中两种有效成分的含量。方法：采用Alltech C18柱,甲醇（A）-0.4%冰醋酸（B）溶液梯度洗脱;检测波长切换法：异嗪皮啶344 nm,五味子醇甲254 nm。结果：线性关系良好,线性范围分别为：0.88-17.6μg/mL,0.76-15.2μg/mL,回收率异嗪皮啶为100.64%,RSD为1.07%;五味子醇甲为100.33%,RSD为0.65%。结论：本测定方法简便可行、重复性好,可用于制剂的质量控制。AIM： To establish a method of simultaneously determining effective components in Ciwujia Naoling Oral Liquid by RP-HPLC. METHODS： Stationary phase： Alltech C18 column, the mobile phase was methanol （A）-0.4% HAC-water （B） with linear gradient elution. Detection was performed at wavelength of 344 nm, 254 nm respectively for isofraxidin and schisandrin. RESULTS： The two effective components could be detected by this method, and their contents could be determined. A good linearity of isofraxidin and schisandrin was in the range of 0.88-17.6 μg/mL, 0.76-15.2 μg/mL respectively. The average recoveries were 100.64%, 100.33% respectively, RSD were 1.07%, 0.65% respectively. CONCLUSION： The method is simple, feasible and reproducible and can be used for the quality control of Ciwujia Naoling Oral Liquid.

关 键 词：HPLC 刺五加脑灵液 异嗪皮啶 五味子醇甲 

分 类 号：R927.2[医药卫生—药学]