转录因子与DNA识别的立体化学规律  

Stereochemical Rules of DNA Recognition by Transcription Factors.

作　　者：杨岐生[1] 

机构地区：[1]浙江大学生物科学与技术系

出　　处：《生物化学与生物物理进展》1998年第1期17-21,共5页Progress In Biochemistry and Biophysics

摘　　要：转录因子ＤＮＡ的识别包含普遍性的化学规律和特异性的立体化学规律．转录因子的识别螺旋以特异性方式结合于ＤＮＡ，位于一侧的大沟内．识别螺旋的“残基连线”和碱基的“碱基连线”之间吻合使两者完全匹配，它通常涉及最多３圈α螺旋和３～５ｂｐ．决定氨基酸残基和碱基相互关系的结合几何图形表示为立体化学图，它表明了识别的特异性．DNA recognition of the transcription factors involves both general chemical rules and specific stereochemical rules. Recognition helice of transcription factor families binds to DNA, positing at a major groove. Pairing between the “residues line” of recognition helix and “base line” of base positions makes full fitting, usually involving 3 turns of αhelix and 3～5 base pairs.The binding geometry determined by interaction of the residues and bases in recognition area is indicated in the stereochemical chart, which shows recognition specificity. Stereochemical rules of DNA recognition by transcription factor family are summarized at bases of the chart.

关 键 词：转录因子 识别 DNA识别 螺旋DNA 立体化学图 

分 类 号：Q75[生物学—分子生物学] Q524