酶放大镧系元素发光法测定碱性磷酸酶  

Enzymeamplified Lanthanide Luminescence Assay of Alkaline Phosphatase

作　　者：赵启仁[1] 李美佳[1] 刘洁[1] 宋娜玲[1] 庄湘莲[1] 陈艾[1] 林汉[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所

出　　处：《生物化学与生物物理进展》1998年第1期71-74,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：报告了应用酶放大镧系元素发光法测定碱性磷酸酶的方法．应用５氟水杨酸磷酸酯作为酶底物，并对方法学中的多种因素进行了最佳比．用甲基硅油（Ｉ）改进了信／噪比的稳定性．方法的灵敏度为４Ｕ／Ｌ．精密度为１０％．精密度为１０％的浓度范围是２００～３００×１０２Ｕ／Ｌ．测量值的相对误差＜１０％．测定了血清中的碱性磷酸酶浓度，回收率为９３％～９５％．An enzymeamplified lanthanide luminescence assay of alkaline phosphatase was described. Optimization of important factors in this assay was studied. Improvement of stability of signal/noise ratio by using methyl silicon oil (I) was first reported. The sensitivity and the accuracy of the assay were 4 U/L and <10% respectively. The concentration range corresponding to 10% precision was 200～302×102 U/L. The activity of alkaline phosphatase in serum was also detected and the recovery was 93%～95%. 5fluorosalicyl phosphate was synthesized and used as substrate.

关 键 词：酶放大 镧系元素 发光分析法 碱性磷酸酶 

分 类 号：Q556.103[生物学—生物化学] Q503