以溶解性可调节高分子为载体的酶免疫分析  

Enzyme Immunoassay Based on Solubility Regulatable Carriers

作　　者：周平[1,2] 曾云鹗[1,2] 邓延倬[1,2] 

机构地区：[1]武汉大学化学系 [2]武汉大学分析测试科学系

出　　处：《生物化学与生物物理进展》1998年第1期79-81,共3页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：将单克隆抗体与溶解性受酸度或温度调节的高分子共价连接．研究了共聚物的溶解性可调节特征，建立了以溶解性可调节高分子为载体酶免疫分析方法，对血清样品中ＨＢｓＡｇ进行了检测，灵敏度０５μｇ／Ｌ，对４３例样品检验的结果与ＥＬＩＳＡ方法相符．Solubleinsoluble forms of monoclonal antibody, which can be rendered either soluble or insoluble by simple adjusting the temperature or pH, have been prepared by covalently coupling antibody to poly(Nisopropylacrylamide) or to copolymer of methacrylic acid and acrylamide. These carriers were used in enzyme immunoassay instead of polystyrenes. Some advantages of both heterogeneous and homogeneous immunoassays were achieved. HBsAg was assayed to examine the sensitivity of these methods. A significant difference from the negative serum was obtained for 05 μg/L. 43 serum samples were assayed, and there was a good correlation between these methods and ELISA.

关 键 词：溶解性 机调节高分子 载体 酶免疫法 

分 类 号：R446.61[医药卫生—诊断学]