门静脉输注诱导耐受中CD4^+T细胞和CD8^+T细胞的不同作用  

Roles of Different Subsets of T Lymphocytes in Portal Venous Injection Induced Tolerance

作　　者：何凡[1] 陈知水[1] 徐胜元[1] 蔡明[1] 吴敏[1] 李红洲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,卫生部/教育部重点实验室,湖北武汉430030

出　　处：《武汉大学学报（医学版）》2009年第3期290-293,F0002,共5页Medical Journal of Wuhan University

基　　金：湖北省科技攻关重大项目(编号:2006AA301A06)

摘　　要：目的:探讨不同T细胞在门静脉输注诱导耐受中的作用。方法:门静脉输注经丝裂霉素处理过的C57小鼠脾细胞给BALB/c,7 d后分离受鼠脾细胞,流式分选其中CD3+、CD4+、CD8+T细胞,过继输注给另一BALB/c,并同时行C57或C3H来源的心脏移植,观察并比较心脏移植物存活时间。结果:过继输注PVI鼠T细胞后,可以转移耐受;过继输注PVI鼠CD4+T细胞,可使供体来源的C57供心存活时间延长,而对第三系C3H供心存活时间无明显延长。过继输注PVI鼠CD8+T细胞无此效应。结论:在门静脉输注诱导耐受的模型中,CD4+T细胞可以转移耐受,且此耐受为抗原特异性,CD8+T细胞无此效应。To analyze the role of different subsets of T lymphocytes in transferring tolerance induced by portal venous injection. Methods： C57BL/6 （B6） mice were used as donors. Seven days after portal venous injection of mitomycin-treated B6 splenocytes into BALB/c mice, the splenocytes of the recipient were isolate, and CD4＋ and CDS＋T lymphocytes were sorted by FACSAria. The sorted CD4＋ （CD8＋ ） T lymphocytes were then transfused into another BALB/c mice followed by heterotopic implantation of a B6 heart allograft. C3H heart allografts and normal BALB/c mice were used as controls. The rejection response and survival of the allograft were observed. Results： When transfusion with CD4＋T tymphocytes isolated from BALB/c mice treated with a portal injection of B6 splenocytes, the subsequent transplanted B6 allograft survived longer than C3H allograft. Similar results were not observed in normal BALB/c or BALB/c mice with transfusion of CD8＋T lymphocytes from BALB/c mice treated with a portal injection of B6 splenocytes. Conclusion： This finding indicates that CD4＋T lymphocytes, rather than CD8＋T lymphocytes, play an important role in transferring tolerance induced by portal venous injection and this tolerance is antigen-specific.

关 键 词：T细胞 门静脉输注 免疫耐受 

分 类 号：R392.4[医药卫生—免疫学]