黄瓜子叶外植体龄对子叶生根的影响  

THE EFFECT OF EXPLANT AGE OF CUCUMBER COTYEDON ON THE ROOTING OF COTYLEDON

作　　者：李伟华[1] 于树宏[1] 王曼[1] 彭斌 

机构地区：[1]华南师范大学生物系

出　　处：《生物学杂志》1998年第1期19-20,23,共3页Journal of Biology

摘　　要：本文对无菌培养条件下黄瓜子叶外植体龄对子叶生根能力的影响进行了研究，结果表明，对于分别萌发５天、１０天、１５天、２０天的子叶在ＭＳ基本培养基上，无论有无生长素ＮＡＡ，均以５天龄的子叶生根能力最强。附加１０ｍｇ／ＬＮＡＡ，显著促进子叶生根。对子叶起始过氧化物酶活性测定表明，子叶起始过氧化物酶活性与生根能力之间存在有一定的关系。The effect of explant age of cucumber cotyledon on the rooting of cotyledon was studied.The results demonstrated that cotyledon aged 5 days rooted best whether there was NAA or not in MS media when compared to the cotyledon aged 10,15 and 20 days,1.0mg/L NAA significantly increased the root number per explant.Initial Peroxidase activity of cotyledon with different age were accordance with their rooting ability.

关 键 词：黄瓜 子叶龄 过氧化物产酶 生根 发育 

分 类 号：S642.201[农业科学—蔬菜学]