家蚕不同地理品种分子系统学研究  

MOLECULAR PHYLOGENETIC STUDY ON THE RACIAL DIFFERENTIATION OF BOMBYX MORI BY RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD) MARKERS

作　　者：夏庆友[1] 周泽扬[1] 鲁成[1] 向仲怀[1] 

机构地区：[1]西南农业大学蚕桑丝绸学院蚕桑学农业部部级重点实验室

出　　处：《昆虫学报》1998年第1期32-40,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金

摘　　要：利用随机引物扩增多态性ＤＮＡ（ＲＡＰＤ）标志，在ＤＮＡ分子水平上对不同系统、化性和眠性共六类５９个家蚕Ｂｏｍｂｙｘｍｏｒｉ品种及野蚕Ｂ．ｍａｎｄａｒｉｎａ之间的遗传差异进行了研究。在３４个随机引物中有１２个（３５．２９％）引物出现稳定的扩增带，扩增总带数为１０３条，其中８６条具多态性，占８３．５％，平均每个引物７．２条。利用单匹配相似系数和ＵＰＧＭＡ聚类法对结果进行分析，发现其ＲＡＰＤ不但具有品种特异性，而且具有系统特异性，证明中国一化性四眠种为最早从野蚕中分化出来的系统，中国一化性三眠种与中国一化性四眠种在进化上属有显著差异的两个类群。The genetic diversity of fiftynine silkworm strains and mulberry wild silkworm, Bombyx mandarina (M.), was investigated at DNA level using the random amplified polymorphic DNA(RAPD) method. Silkworm strains were classified into six groups according to the voltinism, moltinism and the geographic areas initially obtained. Of 34 arbitrary 10base primers that were tested, 12 (3529%) were informative and yielded 103 bands, 86(835%) of which were polymorphic bands with a mean of 72 bands per primer. RAPD data were used to generate simple matching coefficients of similarity (SM). The SM were then used to construct a dendrogram by unweighted pairgroup method with arithmetical averages(UPGMA).The study indicated that RAPD were racespecific and geographic groupspecific markers in silkworm; Chinese univoltine tetramoltine varieties seems to be differentiated form Bombyx mandarina M. in the earliest year; Chinese univoltine varieties should be placed into two distinct groups, i. e., trimoltine varieties and tetramoltine varieties.

关 键 词：家蚕 遗传差异 分子系统树 品种 地理种群 

分 类 号：S882.1[农业科学—特种经济动物饲养]