C型CpG单链寡核苷酸对哮喘小鼠Th1／Th2相关细胞因子的影响  

The effect of type C CpG-ODN on the expression of Th1/Th2 cytokines in a model of asthma

作　　者：陈敏[1] 吴斌[1] 陈雄[1] 

机构地区：[1]广东省湛江广东医学院附属医院呼吸内科,524001

出　　处：《中国临床实用医学》2009年第3期46-48,共3页China Clinical Practical Medicine

摘　　要：目的C型CpG—ODN对哮喘小鼠Th1／Th2相关细胞因子的影响，为CpG-ODN进一步应用提供依据。方法利用人工合成C型的CpG-ODN（ODN-M362），将其注射于哮喘小鼠腹腔，采用酶联免疫吸附法（ELISA）检测注射后小鼠的Th1和Th2细胞因子的含量，分析其有无差异性。结果C型CpG-ODN组BALF中EOS数量，血清IL-4水平明显低于对照组和哮喘组（P〈0．01），血清IFN-γ明显升高于对照组和哮喘组（P〈0．01）。结论C型的CpG-ODN，对哮喘小鼠Th1／Th2失衡有明显的逆转作用。Objective To explore the effect of C types CpG-ODN on the expression of Thl/Th2 cytokines in a model of asthma. Methods Asthmatic mice models were established by ovalbumin intraperitoneal injection and atomization inhalation. Then the mice were intraperitoneally injected with C types CpG-ODN （ ODNM362） ,The serum levels of Th1 cytokine IFN-T and Th2 cytokine IL-4 were measured by ELISA. Meanwhile, eosinophil count peripheral BALF were performed. Results C types CpG-ODN （ODN-M362） inhatitted the expression of Thl cytokine IFN-γ and Th2 cytokine IL-4 in asthmatic mice （P 〈 0. 01 ）. Compared with group A and group B, group C enhanced obviously Thl cytokine IFN-T expression, downregulated Th2 cytokine IL-4 expression. Conclusion ODN-M362, a kind of type C CpG-ODN, could correct the imbalance of Th1/Th2 on cytokine level.

关 键 词：CpG单链寡核苷酸 哮喘 TH1/TH2 

分 类 号：R5[医药卫生—内科学]