透骨草组织培养的研究  

Phryma leptostachya Tissue Culture

作　　者：刘羽[1] 李慧[1] 徐梦[1] 李永德[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《中国林副特产》2009年第2期14-16,共3页Forest By-product and Speciality in China

摘　　要：以透骨草的嫩茎为材料,进行了愈伤组织诱导、愈伤组织分化、不定芽生根、试管苗的移栽、试管苗扦插、试管苗移植的研究,成功的建立起透骨草的嫩茎无性系。结果证明：1/2MS＋BA0.5mg/L＋2,4-D1.2 mg/L＋NAA0.2-0.8 mg/L为嫩茎愈伤组织的的理想培养基;1/2MS＋AgNO31.2 mg/L＋BA0.6 mg/L＋NAA0.1 mg/L为愈伤组织和不定芽分化的理想培养基;1/3MS＋IAA0.4 mg/L为不定芽生根培养和生根继代培养的理想培养基;炉灰渣是试管苗移栽和扦插的理想基质。移植试管苗后期出现生长旺盛、长势整齐的性状,翌年春季出现萌发早5d、根系相当于野生植株2倍的性状。The study on callus induction, differentiation, rooting and transplantation of tube seeding was taken by using young stem of Phryma leptostachya L. var. asiatica Hara as material. The asexual line of young stem was established The results indicated that the ideal medium for induction and differentiation of callus were 1/2MS＋BA 0.5 mg/L ＋2,4-D1.2 mg/L ＋NAA 0.2-0.8 mg/L and 1/2MS＋ AgNO3 1.2 mg/L＋BA 0.6 mg/L ＋NAA 0.1 mg/L respectively. The effective medium for rooting and regeneration was 1/3MS＋IAA 0.4 mg/L. The cinder was the optimum matrix of seedling transplanting. The seedling keeps the exuberant growth.

关 键 词：透骨草 组织培养 无性系 快速繁殖 

分 类 号：S567.202[农业科学—中草药栽培] Q943.1[农业科学—作物学]