柴胡皂甙-d对鸡胚血管形成抑制作用的研究  

Angiogenesis Inhibitory Effect of Saikosaponin-d on Chicken Embryo

作　　者：王宝峰[1] 程延安[2] 党双锁[2] 刘振国[2] 贾晓黎[2] 马红兵[1] 王西京[1] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤科,西安710004 [2]西安交通大学医学院第二附属医院感染科,西安710004

出　　处：《中国中西医结合杂志》2009年第5期425-429,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30471982)

摘　　要：目的观察柴胡皂甙-d(saikosaponin d,SSd)对鸡胚新生血管形成的作用,并探讨其作用的机制。方法制备鸡胚绒毛尿囊膜(chorioallantoic membrane,CAM)模型,86个制备成功,鸡胚开窗后,随机分为4组:SSd大剂量(20μg/mL)组,16个;SSd中剂量(10μg/mL)组,19个;SSd小剂量(5μg/mL)组,25个;对照(PBS,0.01 mol/L)组,26个。将药物或试剂20μL置于CAM表面的载体上。3天后,用数码照相机照像记录血管生长情况;HE染色,普通光镜下观察炎性细胞计数;Leica图像分析系统计算血管生成反应阳性率。结果胚龄第6天时,CAM血管生长呈叶脉状,以载体为中心呈放射状生长,可见血管辐轴,贯穿于载体。加样后SSd各剂量组血管生成明显减少,明胶海绵周围放射状走行的微血管极不明显,可见血管截断、缺如现象。显微镜下SSd各剂量组明胶海绵内及周围CAM间充质内微血管数目及炎性细胞浸润数目均较对照组减少。SSd对中、小血管形成的抑制作用最明显(P<0.05,P<0.01),而对大血管形成的抑制作用较弱,和对照组比较(5.06±1.89vs5.56±2.38),差异无统计学意义(P>0.05)。相关分析显示CAM大血管生成数目与炎性细胞浸润程度未见明显的相关关系(r=0.117,P>0.05);随着炎性细胞浸润数目的增加,CAM小血管生成明显增多,两者呈显著正相关(r=0.971,P<0.01)。结论SSd能够抑制鸡胚生理性血管生成,对中、小血管生成的抑制作用明显,而对大血管形成未见明显的抑制作用,其机制可能与SSd抑制白细胞的游走、活化作用密切相关。Objective To investigate the inhibitory effects of saikosaponin-d （SSd） on angiogenesis in chicken embryos and its mechanism of action. Methods Chorioallantoic membrane （CAM） model was established successfully in 86 chicken embryos. They were divided into 4 groups after fenestration ： the three SSd treated groups （A, B and C） treated with high （20 μg/mL, n=16）, middle （10 μg/mL, n=19） and low （5μg/mL, n =25） dose of SSd respectively, and the control group treated with 0.01 mol/L PBS （ n =26）. The drug or reagent was administered by grafting 20μL onto the surface of CAM. After incubation for 3 days, the vessel growth was recorded by digital photography; inflammatory cells were counted under light microscope with HE staining, and the positive rate of angiogenesis reaction was calculated by Leica image analyzer. Results On the 6th day of the embryonic age, vessels in the chicken embryo CAM showed a radial growing in spok-wheel pattern around the gelatin sponges with lateral axis running through it. Whereas after 3 days of SSd treatment, the angiogenesis reduced significantly with vague microvessels around the sponge, and vascular truncation and absence revealed. Microscopic examinations showed that the number of microvessels and infiltrated inflammatory cells in the sponge and peripheral CAM mesenchyme in the SSd groups were less than those in the control group, especially on vessels of medium and small size （ P 〈 0.05, P 〈 0.01, respectively）, but was insignificant on great vessels （ P 〉 0. 05）. Correlation analysis revealed no correlation between the number of the great vessels in CAM and the infiltrated inflammatory degrees （ r = 0. 117, P 〉 0.05）, but the increase of small vessels in CAM was positively correlated with that of inflammatory cells （ r =0. 971, P 〈 0.01 ）. Conclusions SSd could inhibit the physiological angiogenesis of chicken embryoe, especially for the medium and small vessels, while there was no significant effect on great vessels （ P

关 键 词：柴胡皂甙-D 鸡胚绒毛尿囊膜 血管生成 

分 类 号：R285.5[医药卫生—中药学] R774.1[医药卫生—中医学]