绵羊肌肉IGF-I基因表达的发育性变化研究  

Developmental Changes of IGF-I mRNA Expression Level in Sheep Muscle

作　　者：黄治国[1,2] 谢庄[2] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《安徽农业科学》2009年第14期6375-6377,6415,共4页Journal of Anhui Agricultural Sciences

基　　金：新疆生产建设兵团博士基金(2003-02)

摘　　要：[目的] 研究哈萨克羊和新疆细毛羊肌肉中胰岛素样生长因子Ⅰ（Insulin-like growth factor Ⅰ，IGF-I）基因在发育早期的表达，为绵羊早期生长发育的研究提供资料。[方法]采用实时定量PCR检测2个品种羊在2、30、60、90和120日龄时肌肉中IGF-I mRNA的水平，并用SPSS软件进行统计分析。[结果] 绵羊肌肉IGF-I mRNA的表达量随着日龄的增加呈现出先升后降的趋势，哈萨克羊肌肉IGF-I mRNA的峰值出现于30日龄，新疆细毛羊出现于60日龄哈萨克羊的表达量在2日龄和90日龄时与新疆细毛羊差异不显著（P〉0.05），在30-60日龄期间极显著低于新疆细毛羊（P〈0.01）。[结论] 雄性哈萨克羊和新疆细毛羊肌肉IGF-I mRNA表达具有相似发育性变化模式，但是表达量存在品种间差异。[ Objective ] To detect the mRNA of muscle insulin-like growth factor Ⅰ （IGF-Ⅰ） in the early development of Kazak sheep and Xin- jiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [ Method] With real-time quantitative PCR, the muscle IGF-Ⅰ mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old peaking at 30 days old in kazak sheep and at 60 days old in xinjing fine-wool sheep. Then the data was analyzed with SPSS software. [ Result] The IGF-Ⅰ mRNA of sheep muscle first increased and then decreased with ages. The IGF-Ⅰ expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 days old or 90 days old （P 〉 0. 05 ）, but it was lower than that of the latter with extremely significant difference （P 〈0. 01 ） from 30 to 60 days old. [ Conclusion] The mate Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscle IGF-Ⅰ mRNA, but there are differences in expression level between these two species.

关 键 词：绵羊 IGF-I 表达 

分 类 号：S826[农业科学—畜牧学]