赖氨酸对绵羊GHR基因表达的影响  

Effects of Lysine on the Expression of GHR mRNA in Sheep

作　　者：李建升[1] 程胜利[2] 韩向敏[1] 冯瑞林[2] 岳耀敬[2] 杨博辉[2] 刘建斌[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《安徽农业科学》2009年第14期6381-6382,6424,共3页Journal of Anhui Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF070104)

摘　　要：[目的]研究赖氨酸对绵羊生长的影响及其机理。[方法]选用1岁左右的绵羊15只,均分为A、B、C 3组,分别在基础日粮中添加0、4、10 g赖氨酸盐。饲喂28 d后,全部屠宰,取样。提取组织总RNA,反转录后扩增GHR和GAPDH基因,分析不同处理背最长肌中GHRmRNA的表达丰度。[结果]绵羊GHR基因在B组背最长肌组织中的表达量显著高于对照A组(P<0.01),极显著高于C组(P<0.01);C组与A组间差异不显著(P>0.05)。[结论]在日粮中添加赖氨酸能够提高绵羊GHR基因在背最长肌中的表达量,但不存在剂量依赖性。[ Objective ] This study was to investigate the effects of lysine on the growth sheep and its mechanism. [ Method ] Fifteen one-year old sheep as experimental material were grouped into three groups （group A, group B and group C）, into whose basal feed 0, 4 and 10 g lysine were respectively added. After 28 d of feeding, the experimental sheep were all slaughtered and sampled ; then total RNAs were extracted from the samples and used to clone GHR and GAPDH genes via retrotranscription for analyzing the expression abundance of GHR mRNA in the longest muscle in the back from different treatments. [ Result] The expression of GHR mRNA in treatment B was significantly higher than that in treatment A（CK） at 0. 01 probability level, and significantly higher than that in treatment C at 0. 05 probability level; while that in treatment C and treatment A was insignifieant（P 〉 0.05 ）. [ Conclusion] Addition of lysine into basal feed of sheep could dose-independently improve the expression of GHR gene in the back.

关 键 词：赖氨酸 绵羊 GHR 基因表达 

分 类 号：S826[农业科学—畜牧学]