红斑大壁虎与黑斑大壁虎细胞色素b基因序列差异与分化  

Sequence Variation and Differentiation of Mitochondrial Cytochrome b Gene of Red Gekko gecko and Black Gekko gecko

作　　者：秦新民[1] 钱芳[1] 曾振华[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《安徽农业科学》2009年第14期6383-6384,6402,共3页Journal of Anhui Agricultural Sciences

基　　金：广西环境与保护评价重点实验室项目(桂科能0702Z017);广西教育厅科研项目(200509019)

摘　　要：[目的]探讨黑斑大壁虎和红斑大壁虎(Gekko gecko)之间的分化程度。[方法]对黑斑大壁虎和红斑大壁Cty b基因的序列进行测定,获得Cyt b基因全序列1147 bp。以白脊壁虎(G.vittatus)和沙虎(T.keyserlingii)为外群,用邻接法(NJ)和最大简约法(MP)构建了2种大壁虎的系统发育关系。[结果]黑斑大壁虎与红斑大壁虎Cyt b基因的差异程度达到9.18%,NJ和MP分子系统树显示黑斑大壁虎与红斑大壁虎明显分为二支。[结论]两者达到了亚种分化的差异。[ Objective ] The study aimed to discuss differentiation degree of red Gekko gecko and black Gekko gecko. [ Method ] The complete mitochondrial cytoehrome b genes were sequenced from red C, ekko gecko and black Gekko gecko. 1147 base pairs of G. gecko complete mitochondrial Cyt b gene were sequenced. Taking Gekko vittatus and T. keyserlingii acting as outgroups, phylogenetic relationship between two Gekko geeko was constructed by using NJ and MP methods. [ Result] The phylogenetic trees of the C-ekko gecko were constructed using NJ and MP methods showed that distance was 9.18% between black G. gecko and red G. gecko. [ Conclusion]The genetic difference of black and red G. gecko reached the subspecies differentiation.

关 键 词：大壁虎 遗传分化 细胞色素B基因 

分 类 号：Q953.1[生物学—动物学]