氮离子束注入和钴60伽玛辐射对大豆生物学效应研究初报  

Preliminary Study on Biological Effect of Soybean by Nitrogen Ion Beam Implantation and ^(60)Co-γ Radiation

作　　者：彭琳[1] 季良[1] 

机构地区：[1]新疆农业科学院粮食作物研究所,新疆乌鲁木齐830091

出　　处：《安徽农业科学》2009年第14期6399-6402,共4页Journal of Anhui Agricultural Sciences

基　　金：新疆农业科学院院长基金项目(2005Y04);新疆科技攻关项目(200631104)

摘　　要：[目的]探索氮离子束注入和钴60伽玛辐射对大豆的生物学效应,为大豆诱变育种提供依据。[方法]14份大豆品种或高代稳定品系干种子按4×1016、6×1016和8×1016N+/cm23个剂量和能量30 Kev氮离子束注入,35份大豆品种或高代稳定品系干种子按100、150和200 Gy 3个剂量和剂量率1.6 Gy/Min钴60伽玛辐射,以未处理作对照,考察大豆主要农艺性状和产量,采用近红外透射光谱法测定大豆籽粒品质。[结果]8×1016N+/cm2氮离子束注入和150 Gy钴60伽玛辐射对大豆农艺性状的突变效果明显。氮离子束注入和钴60伽玛辐射诱变蛋白质含量增加的材料占总材料份数的57.1%~78.6%,但6×1016N+/cm2氮离子束注入的蛋白质突变频率大于其余两个剂量,而钴60伽玛辐射剂量间差异不大;诱变脂肪含量增加的材料占总材料份数的34.3%~40.0%,且8×1016N+/cm2氮离子束注入和200 Gy钴60伽玛辐射的脂肪突变频率分别大于其余两个剂量。[结论]选择适宜剂量的氮离子束注入和钴60伽玛辐射可以改善大豆农艺性状,增加产量,提高蛋白质和脂肪含量,因而氮离子束注入和钴60伽玛辐射是两种行之有效的大豆诱变育种方法。[Objective] The study aimed to provide bases for induced mutation breeding on soybean, and discuss the effect of nitrogen ion beam implantation and ^60 Co-γ radiation on biological effect of soybean. [ Method] The dried seeds of 14 soybean varieties or high generation and stable lines were treated by nitrogen ion beam implantation according to 4 × 10^16, 6 ×10^16 and 8 ×10^10N ^＋/cm^2 3 doses and 30 Kev energy, 35 soybean materials were treated with ^60 Co-γ radiation according to 100, 150 and 200 Gy 3 doses and 1.6 Gy/Min dosage ratio, compared with control, observed on main agronomic character and yield of soybean, tested with near-infrared transmission spectroscopy on grain quality of soybean. [ Result] Mutants of agronomic character were evident while the dried seeds of soybean were treated by 8 × 10^16 N ×/cm^2 nitrogen ion beam implantation and 150 Gy ^60 Co-γ radiation. Protein content of soybean grain was more than control with nitrogen ion beam implantation and ^60 Co-γ radiation about 57.1% -78.6% in the total, variation frequency of protein was evidently more than other doses by 6 ×10^16 N ^＋/cm^2 nitrogen ion beam implantation and there were no difference in ^60 Co-γ radiation treatment doses; fat content was more than control by two methods of induced mutation about 34.3% -40.0% in the total, Variation frequency of fat was respectively more than other doses by 8 ×10^16 N ^＋/cm^2nitrogen ion beam implantation and 200 Gy ^60 Co-γ radiation. [ Conclusion ] Selecting the suitable doses of nitrogen ion beam implantation and ^60 Co-γ radiation could improve agronomic character of soybean, increase yield, raise protein and fat content, thus nitrogen ion beam implantation and ^60 Co-γ radiation were effective two methods of induced mutation breeding on soybean.

关 键 词：氮离子束注入 钴60伽玛辐射 大豆 生物学效应 

分 类 号：S565.1[农业科学—作物学]